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Publication Type J
Authors Shen, Y. G., D. Q. Yan, W. K. Zhang, B. X. Du, J. S. Zhang, Q. Liu and S. Y. Chen
Title Novel halophyte EREBP/AP2-type DNA binding protein improves salt tolerance in transgenic tobacco
Source Acta Botanica Sinica
Author Keywords Atriplex hortensis EREBP/AP2-type DNA binding protein transgenic tobacco s-adenosylmethionine decarboxylase oryza-sativa l. gene- expression low-temperature transcription factors signal- transduction rice seedlings osmotic-stress drought arabidopsis
Abstract EREBP/AP2-type proteins are members of a large DNA binding protein (DBP) family found in plants. Some members like APETALA2 and AtDREB/CBF can regulate flower development and response to environmental stresses, respectively. To characterize transcription factors involved in plant responses to salt stress, we constructed cDNA library from salt-treated halophyte (Atriplex hortensis) and isolated a novel gene encoding EREBP/AP2-type protein from this library. This cDNA contained an ORF of 723 bp and a long 3'-Untranslated-Region (UTR) of 655 bp. The deduced amino acid sequence showed one conserved DNA binding domain of EREBP/AP2, thus the corresponding gene was named AhDREB1 with a calculated molecular mass of 26.1 kD. AhDREB1 under the control of CaMV 35S promoter was then transformed into tobacco and nine independent transgenic lines were obtained and subjected to long term salt stress. The results suggested that overexpression of AhDREB1 improved the salt tolerance in transgenic tobacco through functioning as a regulatory molecule in response to salt stress. Analysis of Arabidopsis genome in database resulted in dozens of EREBP/AP2-type homologous proteins, of which seven members showed high similarity to AhDREB1. Secondary structure analysis predicted similar arrangement of a-helix in their DNA binding domains.
Author Address Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China NW Sci Technol Univ Agr & Forestry, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China Liu Q Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
29-Character Source Abbreviation Acta Bot. Sin.
Publication Date Jan
Year Published 2003
Volume 45
Issue 1
Beginning Page 82-87
Unique Article Identifier ISI:000180476600012
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