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Publication Type J
Authors Saleh, B.
Title R-ISSR marker as a useful tool for detection of new genomic loci in Arthrocnemum macrostachyum
Source Biologia Plantarum
Author Keywords PCR polymorphism RAPD genetic diversity rapd polymorphism cultivars oryza
Abstract Arthrocnemum macrostachyum, is a perennial halophytic shrub typical of Mediterranean salt marshes. The present study aims to investigate some combinations of inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) primers applied in real PCR. Thereby, the potential of R-ISSR markers to detect new genomic loci in 3 genotypes of A. macrostachyum grown in the Western coast of Syria was examined. Different combinations of RAPD and ISSR primers produced bands that were absent when single ISSR or RAPD primers were used. The results have demonstrated that ISSR primer (AG)(8)TC gave more informative pattern when combined with different RAPD primers comparing to other tested primers. In contrast, the tested ISSR primer (GACA)(4) gave less informative pattern when used alone. These combinations were successfully applied in real PCR to detect new genomic variability in A. macrostachyum genotypes.
Author Address AECS, Dept Mol Biol & Biotechnol, Damascus, Syria. Saleh, B (reprint author), AECS, Dept Mol Biol & Biotechnol, POB 6091, Damascus, Syria. scientific@aec.org.sy
ISSN 0006-3134
ISBN 0006-3134
29-Character Source Abbreviation Biol. Plant.
Publication Date Jun
Year Published 2011
Volume 55
Issue 2
Beginning Page 327-330
Unique Article Identifier WOS:000293030600017
Plants associated with this reference

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