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Publication Type J
Authors Rivoal, J. and A. D. Hanson
Title CHOLINE-O-SULFATE BIOSYNTHESIS IN PLANTS - IDENTIFICATION AND PARTIAL CHARACTERIZATION OF A SALINITY-INDUCIBLE CHOLINE SULFOTRANSFERASE FROM SPECIES OF LIMONIUM (PLUMBAGINACEAE)
Source Plant Physiology
Author Keywords BOMBARDMENT MASS-SPECTROMETRY SALT TOLERANCE BETAINE SPINACH PURIFICATION CHLOROPLASTS TOBACCO STRESS Plant Sciences
Abstract Choline-O-sulfate is a compatible osmolyte accumulated under saline conditions by members of the halophytic genus Limonium and other Plumbaginaceae. A choline sulfotransferase (EC 2.8.2.6) responsible for the formation of choline-O-sulfate was characterized in Limonium species. A simple radiometric assay was developed in which [C-14]choline was used as substrate, and the h [C-14]choline-O-sulfate product was isolated by ion-exchange chromatography. The choline sulfotransferase activity was soluble, required 3'-phosphoadenosine-5'-phosphosulfate as the sulfate donor, and showed a pH optimum at 9.0. Apparent K-m values were 25 mu M for choline and 5.5 mu M for 3'-phosphoadenosine-5'-phosphosulfate. Choline sulfotransferase activity was detected in various Limonium species but was very low or absent from species that do not accumulate choline-O-sulfate. In roots and leaves of Limonium perezii, the activity was increased at least 4-fold by salinization with 40% (v/v) artificial sea water. Choline sulfotransferase activity was also induced in cell cultures of L. perezii following salt shock with 20% (v/v) artificial sea water or osmotic shock with 19% (w/v) polyethylene glycol 6000. Labeling experiments with [C-14]choline confirmed that the enzyme induced in cell cultures was active in vivo.
Author Address UNIV MONTREAL,INST RECH BIOL VEGETALE,MONTREAL H1X 2B2,PQ,CANADA.
ISSN 0032-0889
ISBN 0032-0889
29-Character Source Abbreviation Plant Physiol.
Publication Date Nov
Year Published 1994
Volume 106
Issue 3
Beginning Page 1187-1193
Unique Article Identifier WOS:A1994PT65400045
Plants associated with this reference

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