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Publication Type J
Authors Prinz, K., S. Schie, T. Debener, I. Hensen and K. Weising
Title Microsatellite markers for Spergularia media (L.) C. Presl. (Caryophyllaceae) and their cross-species transferability
Source Molecular Ecology Resources
Author Keywords halophytes microsatellites Spergularia media SSRs
Abstract We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross-amplified in the closely related S. salina. Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra.
Author Address [Prinz, Kathleen; Weising, Kurt] Univ Kassel, D-34109 Kassel, Germany. [Hensen, Isabell] Univ Halle Wittenberg, Inst Biol Geobot, Halle, Germany. [Hensen, Isabell] Univ Halle Wittenberg, Bot Garden, Halle, Germany. [Schie, Stephan; Debener, Thomas] Leibniz Univ Hannover, Inst Plant Genet, Hannover, Germany. Weising, K, Univ Kassel, D-34109 Kassel, Germany. weising@uni-kassel.de
ISSN 1755-098X
ISBN 1755-098X
29-Character Source Abbreviation Mol. Ecol. Resour.
Publication Date Sep
Year Published 2009
Volume 9
Issue 5
Beginning Page 1424-1426
Digital Object Identifier (DOI) 10.1111/j.1755-0998.2009.02680.x
Unique Article Identifier ISI:000268855000028
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