Loading content, please wait..
loading..
Logo
Version 3.22
or
Publication Type J
Authors Nishiuchi, S., K. Fujihara, S. K. Liu and T. Takano
Title Analysis of expressed sequence tags from a NaHCO(3)-treated alkali-tolerant plant, Chloris virgata
Source Plant Physiology and Biochemistry
Author Keywords cDNA library Chloris virgata Expressed sequence tags Gene ontology annotation NaHCO(3) stress Real-time RT-PCR functional genomics salinity tolerance cold tolerance abiotic stress cdna library arabidopsis salt halophyte gene identification
Abstract Chloris virgata Swartz (C. virgata) is a gramineous wild plant that can survive in saline-alkali areas in northeast China. To examine the tolerance mechanisms of C virgata, we constructed a cDNA library from whole plants of C. virgata that had been treated with 100 mM NaHCO(3) for 24 h and sequenced 3168 randomly selected clones. Most (2590) of the expressed sequence tags (ESTs) showed significant similarity to sequences in the NCBI database. Of the 2590 genes, 1893 were unique. Gene Ontology (GO) Slim annotations were obtained for 1081 ESTs by BLAST2GO and it was found that 75 genes of them were annotated with GO terms "response to stress", "response to abiotic stimulus", and "response to biotic stimulus", indicating these genes were likely to function in tolerance mechanism of C virgata. In a separate experiment, 24 genes that are known from previous studies to be associated with abiotic stress tolerance were further examined by real-time RT-PCR to see how their expressions were affected by NaHCO(3) stress. NaHCO(3) treatment up-regulated the expressions of pathogenesis-related gene (DC998527), Win1 precursor gene (DC998617), catalase gene (DC999385), ribosome inactivating protein 1 (DC999555), Na(+)/H(+) antiporter gene (DC998043), and two-component regulator gene (DC998236). (c) 2010 Elsevier Masson SAS. All rights reserved.
Author Address [Nishiuchi, S; Fujihara, K; Takano, T] Univ Tokyo, ANESC, Lab Environm Stress Tolerance Mech, Nishitokyo, Tokyo 1880002, Japan [Liu, SK] NE Forestry Univ, Stress Mol Biol Lab, ASNESC, Harbin 150040, Peoples R China Takano, T (reprint author), Univ Tokyo, ANESC, Lab Environm Stress Tolerance Mech, 1-1-1 Midori Cho, Nishitokyo, Tokyo 1880002, Japan takano@anesc.u-tokyo.ac.jp
ISSN 0981-9428
ISBN 0981-9428
29-Character Source Abbreviation Plant Physiol. Biochem.
Publication Date Apr
Year Published 2010
Volume 48
Issue 4
Beginning Page 247-255
Digital Object Identifier (DOI) 10.1016/j.plaphy.2010.01.024
Unique Article Identifier WOS:000276803200006
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed