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Publication Type J
Authors Megdiche, W., C. Passaquet, W. Zourrig, Y. Z. Fodil and C. Abdelly
Title Molecular cloning and characterization of novel cystatin gene in leaves Cakile maritima halophyte
Source Journal of Plant Physiology
Author Keywords Cakile maritima Cystatin Environmental stresses RACE Variability CYSTEINE PROTEINASE-INHIBITOR ANIMAL CYSTATINS COLD-STRESS RICE SEEDS CELL-DEATH EXPRESSION ORYZACYSTATIN PLANT ARABIDOPSIS SALT
Abstract Cakile maritima (Brassicaceae) is a halophyte that thrives on dunes along Mediterranean seashores, with high tolerance to salty and dry environments. We have previously shown that there is great morphological and physiological diversity between ecotypes. We investigated the expression of cysteine protease inhibitor (cystatin) genes in the response to hydric and saline constraints, as cystatins are known to participate in the response to environmental constraints in plants. We isolated, from C. maritime, a new cystatin cDNA (CmC) that encodes a 221 amino acid protein with a calculated molecular mass of 25 kDa. It displays a moderate-to-high amino acid sequence similarity with previously reported phytocystatin genes. The predicted protein is hydrophilic, with only one hydrophobic region, just at its N-terminus, and a calculated isoelectric point of 6.7. Sequence analysis revealed a monocystatin structure with one cystatin-like domain. The predicted protein CmC contains the main conserved motifs characteristic of the plant cystatins, and a putative site of phosphorylation by casein kinase II (TPSD). As some cystatins, it contains a C-terminal extension of 106 amino acid residues, with several conserved cystatin motifs. The expression was constitutive in non-stressed plants, with different levels between the ecotypes, and without apparent relation to the climatic area of origin. Augmented expression was observed under severe salinity except in the ecotype from the and region. Water deficit also increased CmC expression in two ecotypes, with the highest value observed in the ecotype from the humid region. These results indicate that C. maritima responds to high salinity and water deficit by
Author Address [Megdiche, Wided; Zourrig, Walid; Abdelly, Chedly] Ctr Biotechnol Technopole Borj Cedria CBBC, Lab Adaptat Plantes Stress Abiot, Hammam Lif 2050, Tunisia. [Passaquet, Chantal; Fodil, Yasmine Zuily] Univ Paris 12, F-94010 Creteil, France. Megdiche, W, Ctr Biotechnol Technopole Borj Cedria CBBC, Lab Adaptat Plantes Stress Abiot, BP 901, Hammam Lif 2050, Tunisia. ksouriwided@yahoo.fr
ISSN 0176-1617
ISBN 0176-1617
29-Character Source Abbreviation J. Plant Physiol.
Year Published 2009
Volume 166
Issue 7
Beginning Page 739-749
Digital Object Identifier (DOI) 10.1016/j.jplph.2008.09.012
Unique Article Identifier ISI:000265461500007
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