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Publication Type J
Authors Lokhande, V. H., T. D. Nikam, V. Y. Patade and P. Suprasanna
Title Morphological and molecular diversity analysis among the Indian clones of Sesuvium portulacastrum L
Source Genetic Resources and Crop Evolution
Language English
Author Keywords Ac homologous region ITS Morphological and molecular diversity RAPD Sesuvium portulacastrum RIBOSOMAL DNA RAPD MARKERS PHYLOGENY MANGROVES POLYMORPHISM GENE IDENTIFICATION POPULATIONS EVOLUTION SEQUENCES
Abstract Sesuvium portulacastrum L. (seapurslane) is a halophyte used as pioneer species in sand dune fixation and stabilization of saline soil. Studies on the morphological and molecular diversity were carried out for the 14 clones of Sesuvium collected from the different coastal regions of India. Significant differences were observed for morphological traits viz., length, width, diameter and area of leaf, internodal distance and stem diameter for different clones when compared with the clone from Gujarat state (GJ1). A UPGMA dendrogram for morphological traits based on the Pearson's similarity coefficient clustered the clones into three groups considering 80% polymorphism as criteria. Molecular diversity among the clones was studied using Randomly Amplified Polymorphic DNA (RAPD), Internal Transcribed Spacer (ITS) and markers specific to Ac homologous region. Of the total 749 RAPD loci amplified with 70 random primers, 294 were polymorphic with 39.25% diversity. A phylogenetic tree constructed with UPGMA and SHAN, grouped the clones into three major clades based on RAPD data. The molecular diversity studied with ITS and markers specific to Ac homologous region revealed 37.50% and 66.66% polymorphism and clustered the clones into three and four clades, respectively. The genetic diversity analysis revealed wide variations among the S. portulacastrum clones, reflecting a high level of diversity within the species which might be due to anthropogenic impact and geographic environmental conditions. Further, the various clones from the different eco-geographic coastal localities might have originated from native places of wild abundance. To the best of our knowledge, this is the first attempt to evaluate both morphological and genetic diversity among the Sesuvium clones collected from the distant habitats of the coastal regions of the India.
Author Address [Patade, Vikas Y.; Suprasanna, Penna] Bhabha Atom Res Ctr, Funct Plant Biol Sect, Nucl Agr & Biotechnol Div, Mumbai 400085, Maharashtra, India. [Lokhande, Vinayak H.; Nikam, Tukaram D.] Univ Poona, Dept Bot, Pune 411007, Maharashtra, India. Suprasanna, P, Bhabha Atom Res Ctr, Funct Plant Biol Sect, Nucl Agr & Biotechnol Div, Mumbai 400085, Maharashtra, India. prasanna@barc.gov.in
ISSN 0925-9864
ISBN 0925-9864
29-Character Source Abbreviation Genet. Resour. Crop Evol.
Publication Date Aug
Year Published 2009
Volume 56
Issue 5
Beginning Page 705-717
Digital Object Identifier (DOI) 10.1007/s10722-008-9396-9
Unique Article Identifier ISI:000267486000011
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