Loading content, please wait..
loading..
Logo
Version 3.22
or
Publication Type J
Authors Lan, T., Y. L. Duan, B. Wang, Y. C. Zhou and W. R. Wu
Title Molecular cloning and functional characterization of a Na(+)/H(+) antiporter gene from halophyte Spartina anglica
Source Turkish Journal of Agriculture and Forestry
Author Keywords Halophyte salt tolerance SaNHX1 Spartina anglica transgenic rice salt-stress arabidopsis-thaliana transcription factors tonoplast vesicles transgenic rice abscisic-acid expression tolerance drought overexpression
Abstract Na(+)/H(+) antiporters have been suggested to play important roles in salt tolerance in plants. English cordgrass (Spartina anglica) is a gramineous halophyte with very strong salt tolerance. It possesses salt glands in its stems and leaves, through which excess salt can be excreted. In this study, a vacuolar Na(+)/H(+) antiporter homologous gene (temporarily named SaNHX1) was isolated from Spartina anglica by RT-PCR and rapid amplification of cDNA ends (RACE). Southern blot analysis suggested that there might be 2 or 3 copies of the vacuolar Na(+)/H(+) antiporter genes in the English cordgrass genome. Northern blot analysis showed that the expression of vacuolar Na(+)/H(+) antiporter genes in English cordgrass is induced by salt stress. Overexpression of SaNHX1 driven by constitutive promoter Ubi-1 in rice significantly enhanced the salt tolerance of transgenic plants, validating the function of SaNHX1 and suggesting its value for the genetic improvement of salt tolerance in plants.
Author Address [Lan, T; Duan, YL; Wang, B; Zhou, YC; Wu, WR] Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab, Fuzhou 350002, Fujian, Peoples R China. [Lan, T; Duan, YL; Zhou, YC; Wu, WR] Fujian Prov Key Lab Rice Mol Marker Assisted Bree, Fuzhou 350002, Fujian, Peoples R China. [Lan, T; Duan, YL; Zhou, YC] Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Fujian, Peoples R China. [Wang, B; Wu, WR] Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Fujian, Peoples R China. Wu, WR (reprint author), Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab, Fuzhou 350002, Fujian, Peoples R China wuwr@fiau.edu.cn
ISSN 1300-011X
ISBN 1300-011X
29-Character Source Abbreviation Turk. J. Agric. For.
Year Published 2011
Volume 35
Issue 5
Beginning Page 535-543
Digital Object Identifier (DOI) 10.3906/tar-1003-2
Unique Article Identifier WOS:000296111800009
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed