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Publication Type J
Authors Kluge, C., P. Lamkemeyer, N. Tavakoli, D. Golldack, A. Kandlbinder and K. J. Dietz
Title cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress
Source Molecular Membrane Biology
Author Keywords V-ATPase; M. crystallinum; salt stress; vha; expression analysis vacuolar h+-atpase; trans-complex formation; translocating atpase; mutational analysis; higher-plant; salt stress; sequence; growth; roots; arabidopsis
Abstract The vacuolar-type ATPase (V-ATPase) and the vacuolar H+- pyrophosphatase are electrogenic proton pumps at plant endomembranes that create the proton motive force required for secondary activated transport and metabolite accumulation during development and adaptation to a variety of adverse growth conditions. Twelve distinct vacuolar H+-ATPase (VHA) subunits are suggested to constitute the functional V-ATPase complex. Starting from the available expressed sequence tag ( EST) sequences and by homology screening, the complete set of 12 VHA subunits was cloned as cDNAs from the halophyte Mesembryanthemum crystallinum, vha-A-H, - a, - c, - d and - e. Transcript levels of all 12 VHA subunits as well as of tonoplast pyrophosphatase and P-ATPase were analysed in root and leaf tissue under conditions of osmotic (700 mM mannitol), heat and cold stress, and salinity. Distinct coordinated changes of stress-induced expression were observed for most subunits in roots and leaves, with mostly paralleled changes in transcript levels of all subunits. In some cases, contrasting responses were seen for vha-B and - c transcript amounts.
Author Address Univ Bielefeld, Dept Physiol & Biochem Plants, W5, D-33501 Bielefeld, Germany Univ Bielefeld, Dept Physiol & Biochem Plants, D-33501 Bielefeld, Germany Dietz KJ Univ Bielefeld, Dept Physiol & Biochem Plants, W5, D-33501 Bielefeld, Germany
29-Character Source Abbreviation Mol. Membr. Biol.
Publication Date Apr
Year Published 2003
Volume 20
Issue 2
Beginning Page 171-183
Unique Article Identifier ISI:000183653200009
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