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Publication Type J
Authors Kalsoom, U., M. C. Boyce, I. J. Bennett and V. Veraplakorn
Title Simultaneous Determination of Key Osmoregulants in Halophytes Using HPLC-ELSD
Source Chromatographia
Author Keywords Column liquid chromatography ELSD Betaine Mannitol Proline Osmoregulators performance liquid-chromatography pantoea-agglomerans cpa-2 glycine-betaine amino-acids mass-spectrometry proline analogs plant-extracts water-stress accumulation salt
Abstract Osmoregulants are the substances produced by plants that assist in tolerating environmental stresses. Three commonly analysed osomoregulants include mannitol, betaine and proline. A simple, sensitive and rapid HPLC-ELSD method has been developed for the simultaneous analysis of these common osmoregulants in plant extracts. Osmoregulants were extracted using 80 % ethanol and separated on an NH2 column using 0.1 % formic acid and acetonitrile as the mobile phase. Retention time repeatability was 0.85, 1.50, and 0.93 % for mannitol, betaine and proline, respectively. The limit of detection (mu mol) was 1.43 x 10(-4), 7.81 x 10(-5) and 1.08 x 10(-4) for mannitol, betaine and proline, respectively. The developed method was applied to three different plant extracts, Stylosanthes guianensis, Atriplex cinerea and Rhagodia baccata. A second method using a C18 column with 0.1 % heptafluorobutyric acid and acetonitrile as the mobile phase proved to be a useful complementary method for verifying tentative peak identifications.
Author Address [Kalsoom, Umme; Boyce, Mary C.; Bennett, Ian J.] Edith Cowan Univ, Sch Nat Sci, Perth, WA 6027, Australia. [Veraplakorn, Varaporn] Ramkhamhang Univ, Fac Sci, Dept Biotechnol, Bangkok 10240, Thailand. Boyce, MC (reprint author), Edith Cowan Univ, Sch Nat Sci, Perth, WA 6027, Australia. m.boyce@ecu.edu.au
ISSN 0009-5893
ISBN 0009-5893
29-Character Source Abbreviation Chromatographia
Publication Date Sep
Year Published 2013
Volume 76
Issue 17-18
Beginning Page 1125-1130
Digital Object Identifier (DOI) 10.1007/s10337-013-2494-7
Unique Article Identifier WOS:000323373400008
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