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Publication Type J
Authors Hessini, K., K. Ben Hamed, M. Gandour, M. Mejri, C. Abdelly and C. Cruz
Title Ammonium nutrition in the halophyte Spartina alterniflora under salt stress: evidence for a priming effect of ammonium?
Source Plant and Soil
Author Keywords Ammonium, antioxidant enzymes Nitrate Salt stress Spartina alterniflora different nitrogen-sources antioxidant responses superoxide-dismutase hydrogen-peroxide oxidative stress higher-plants salinity seedlings toxicity tolerance
Abstract The effects of salt stress on the salt marsh halophyte Spartina alterniflora have been well documented. However, plant responses to combined salinity and ammonium toxicity and the underlying mechanisms are relatively unknown. The aim of the present investigation was to study the effects of both salinity (0, 200 and 500 mM NaCl) and nitrogen form (NO3 (-), NH4 (+) or NH4NO3) on S. alterniflora. Plants were cultivated in sandy soil under greenhouse conditions for 3 months. At harvest, growth parameters were measured and leaf samples were analysed for oxidative stress parameters (malondialdehyde, MDA; electrolyte leakage, EL; and hydrogen peroxide, H2O2 concentration) and the activity of antioxidant enzymes (glutathione reductase, GR; superoxide dismutase, SOD; catalase, CAT; ascorbate peroxidase, APX and Guaiacol peroxidase, GPX). In the absence of NaCl, plant growth rate was the highest in the medium containing both nitrogen forms, and the lowest in the medium containing only nitrate. Irrespective of the nitrogen form, plant growth was generally higher at 200 mM NaCl than without salinity. Ammonium-fed plants showed better growth than nitrate-fed plants under high salinity. In the absence of salinity, ammonium-fed plants showed higher SOD, APX, GR, CAT, and GPX activities than nitrate-fed ones. The antioxidant enzymes exhibited higher activity in saline-treated plants. The considerable advantage of NH4 (+) nutrition to S. alterniflora under saline conditions was associated with high antioxidant enzyme activities, together with low MDA content, EL, and H2O2 concentration. These data clearly demonstrate that NH4 (+) is more favourable for the growth of S. alterniflora under high salinity than NO3 (-). It is suggested that NH4 (+) nutrition improves the plant's capacity to limit oxidative damage by stimulating the activities of the major antioxidant enzymes.
Author Address [Hessini, Kamel] Ctr Biotechnol, Lab Physiol Mol Plantes, Hammam Lif 2050, Tunisia. [Ben Hamed, Karim; Gandour, Mhemmed; Mejri, Maroua; Abdelly, Chedly] Ctr Biotechnol, Lab Plantes Extremophiles, Hammam Lif 2050, Tunisia. [Cruz, Cristina] Univ Lisbon, Fac Ciencias, Dept Biol Vegetal, CBA, P-1749016 Lisbon, Portugal. Hessini, K (reprint author), Ctr Biotechnol, Lab Physiol Mol Plantes, Technopole Borj Cedria,PB 901, Hammam Lif 2050, Tunisia. hessini_kamel@yahoo.fr
ISSN 0032-079X
ISBN 0032-079X
29-Character Source Abbreviation Plant Soil
Publication Date Sep
Year Published 2013
Volume 370
Issue 1-2
Beginning Page 163-173
Digital Object Identifier (DOI) 10.1007/s11104-013-1616-1
Unique Article Identifier WOS:000323253500012
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