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Publication Type J
Authors Hanson, A. D., B. Rathinasabapathi, B. Chamberlin and D. A. Gage
Title Comparative Physiological Evidence That Beta-Alanine Betaine and Choline-O-Sulfate Act as Compatible Osmolytes in Halophytic Limonium Species
Source Plant Physiology
Author Keywords glycine betaine; quaternary ammonium; escherichia-coli; sugar-beet; pathway; salinity; spinach
Abstract The quaternary ammonium compounds accumulated in saline conditions by five salt-tolerant species of Limonium (Plumbaginaceae) were analyzed by fast atom bombardment mass spectrometry. Three species accumulated beta-alanine betaine and choline-O-sulfate; the others accumulated glycine betaine and choline-O-sulfate. Three lines of evidence indicated that beta-alanine betaine and choline-O-sulfate replace glycine betaine as osmoregulatory solutes. First, tests with bacteria showed that beta-alanine betaine and choline-O-sulfate have osmoprotective properties comparable to glycine betaine. Second, when beta-alanine betaine and glycine betaine accumulators were salinized, the levels of their respective betaines, plus that of choline-O-sulfate, were closely correlated with leaf solute potential. Third, substitution of sulfate for chloride salinity caused an increase in the level of choline-O-sulfate and a matching decrease in glycine betaine level. Experiments with C-14-labeled precursors established that beta-alanine betaine accumulators did not synthesize glycine betaine and vice versa. These experiments also showed that beta-alanine betaine synthesis occurs in roots as well as leaves of beta-alanine betaine accumulators and that choline-O-sulfate and glycine betaine share choline as a precursor. Unlike glycine betaine, beta-alanine betaine synthesis cannot interfere with conjugation of sulfate to choline by competing for choline and does not require oxygen. These features of beta-alanine betaine may be advantageous in sulfate-rich salt marsh environments.
Author Address MICHIGAN STATE UNIV,DEPT ENERGY,PLANT RES LAB,E LANSING,MI 48824. MICHIGAN STATE UNIV,DEPT BIOCHEM,E LANSING,MI 48824. HANSON, AD, UNIV MONTREAL,INST RECH BIOL VEGETALE,4101 RUE SHERBROOKE EST,MONTREAL H1X 2B2,QUEBEC,CANADA.
29-Character Source Abbreviation Plant Physiol.
Publication Date Nov
Year Published 1991
Volume 97
Issue 3
Beginning Page 1199-1205
Unique Article Identifier ISI:A1991GT79900048
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