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Publication Type J
Authors Guo, Y. Q., Z. Y. Tian, G. Y. Qin, D. L. Yan, J. Zhang, W. Z. Zhou and P. Qin
Title Gene expression of halophyte Kosteletzkya virginica seedlings under salt stress at early stage
Source Genetica
Author Keywords cDNA-AFLP Gene expression Kosteletzkya virginica (L.) Presl. Salt stress ACID BETA-OXIDATION ARABIDOPSIS-THALIANA TRANSCRIPTION FACTOR SIGNAL-TRANSDUCTION MESEMBRYANTHEMUM-CRYSTALLINUM FACULTATIVE HALOPHYTE TRANSGENIC PLANTS DROUGHT TOLERANCE NA+ TRANSPORT AFLP ANALYSIS
Abstract Gene differential expression of Kosteletzkya virginica seedlings under salt stress at two time points (2, 24 h) in roots and leaves was analyzed using the cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique. Polymorphic transcript-derived fragments (TDFs) among control plants and salt-treated plants were grouped into four main differential expression patterns: repression (A), de novo induction (B), up-regulation (C) and down-regulation (D). Among them, 34 differentially expressed gene fragments were homologous to known genes from other species and 4 were sequences with unknown functions. These differentially expressed genes can be classified into four groups according to their putative functions: (1) genes for re-establishing ion homeostasis and protecting the plant from stress damage; (2) genes involved in metabolism or energy and resuming plant growth and development under salt stress; (3) genes involved in regulation of gene expression; (4) genes for signal transduction. Changes of eight differentially expressed genes were confirmed by quantitative real time RT-PCR.
Author Address [Guo, Yu-Qi; Yan, Dao-Liang; Zhang, Jie; Zhou, Wen-Zong; Qin, Pei] Nanjing Univ, Halophyte Res Lab, Nanjing 210093, Peoples R China. [Guo, Yu-Qi; Tian, Zeng-Yuan; Qin, Guang-Yong] Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Peoples R China. Qin, P, Nanjing Univ, Halophyte Res Lab, Nanjing 210093, Peoples R China. Qinpei@nju.edu.cn
ISSN 0016-6707
ISBN 0016-6707
29-Character Source Abbreviation Genetica
Publication Date Nov
Year Published 2009
Volume 137
Issue 2
Beginning Page 189-199
Digital Object Identifier (DOI) 10.1007/s10709-009-9384-9
Unique Article Identifier ISI:000270431400008
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