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Publication Type J
Authors Guan, B., Y. Z. Hu, Y. L. Zeng, Y. Wang and F. C. Zhang
Title Molecular characterization and functional analysis of a vacuolar Na+/H+ antiporter gene (HcNHX1) from Halostachys caspica
Source Molecular Biology Reports
Author Keywords Na+/H+ antiporter Salt tolerance Halostachys caspica HcNHX1 Transgenic plant wheat triticum-aestivum zea-mays l. salt tolerance arabidopsis-thaliana thellungiella-halophila salinity tolerance suaeda-salsa stress expression cloning
Abstract According to sequences of several vacuolar Na+/H+ antiporter genes from Xinjiang halophytic plants, a new vacuolar Na+/H+ antiporter gene (HcNHX1) from the halophyte Halostachys caspica was obtained by RACE and RT-PCR using primers corresponding to conserved regions of the coding sequences. The obtained HcNHX1 cDNA was 1,983 bp and contained a 1,656 bp open reading frame encoding a deduced protein of 551 amino acid residues. The deduced amino acid sequence showed high identity with other NHX1 we have cloned previously from halophyte in Xinjiang desert area. The phylogenetic analysis showed that HcNHX1 formed a clade with NHX homologs of Chenopodiaceae. Expression profiles under salt treatment and ABA induction were investigated, and the results revealed that expression of HcNHX1 was induced by NaCl and ABA. To compare the degree of salt tolerance, we over-expressed HcNHX1 in Arabidopsis. Two transgenic lines grew more vigorously than the wild type (WT) under salt stress. The analysis of ion contents indicated that under salt stress, the transgenic plants compartmentalized more Na+ in the leaves compared with wild-type plants. Together, these results suggest that the products of the novel gene HcNHX1 from halophyte Halostachys caspica is a functional tonoplast Na+/H+ antiporter.
Author Address [Guan, Bo; Hu, Youzhen; Zeng, Youling; Wang, Yan; Zhang, Fuchun] Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China. Zhang, FC (reprint author), Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China. gbb3519@yahoo.com.cn; zfcxju@gmail.com
ISSN 0301-4851
ISBN 0301-4851
29-Character Source Abbreviation Mol. Biol. Rep.
Publication Date Mar
Year Published 2011
Volume 38
Issue 3
Beginning Page 1889-1899
Digital Object Identifier (DOI) 10.1007/s11033-010-0307-8
Unique Article Identifier WOS:000286941400057
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