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Publication Type J
Authors Diedhiou, C. J., O. V. Popova and D. Golldack
Title Transcript profiling of the salt-tolerant Festuca rubra ssp litoralis reveals a regulatory network controlling salt acclimatization
Source Journal of Plant Physiology
Author Keywords Aquaporin Festuca rubra ssp litoralis Na(+)/H(+) antiporter Salt stress Vacuolar H(+)-ATPase vacuolar h+-atpase dependent protein-kinase na+/h+ antiport activity mesembryanthemum-crystallinum arabidopsis-thaliana induced expression osmotic-stress high salinity ice plant roots
Abstract We report an analysis of salt-stress responses in the monocotyledonous halophyte Festuca rubra ssp. litoralis. Salt-dependent expression of transcripts encoding a PIP2;1 aquaporin, V-ATPase subunit B, and the Na(+)/H(+) antiporter NHX was characterized. Transcription of FrPIP2;1, FrVHA-B, and FrNHX1 was induced in root tissue of F rubra ssp. litoralis by salt treatment, and during salt-stress F rubra ssp. litoralis accumulated sodium in leaves and roots. Cell specificity of FrPIP2;1, FrVHA-B, and FrNHX1 transcription was analyzed by in situ PCR in roots of F rubra ssp. litoralis. Expression of the genes was localized to the root epidermis, cortex cells, endodermis, and the vascular tissue. In plants treated with 500 mM NaCl, transcripts were repressed in the epidermis and the outer cortex cells' whereas endodermis and vasculature showed strong signals. These data demonstrate that transcriptional regulation of the aquaporin PIP2;1, V-ATPase, and the Na(+)/H(+) antiporter NHX is correlated with salt tolerance in F rubra ssp. litoralis and suggests coordinated control of ion homeostasis and water status at high salinity in plants. Salt-induced transcript accumulation in F rubra ssp. litoralis was further monitored by cDNA-arrays with expressed sequence tags derived from a cDNA subtraction library. The salt-regulated transcripts included those involved in the control of gene expression and signal transduction elements such as a serine/threonine protein kinase, an SNF1-related protein kinase, and a WRKY-type transcription factor. Other ESTs with salt-dependent regulation included transcripts encoding proteins that function in metabolism, general stress responses, and defense and transport proteins. (C) 2008 Elsevier GmbH. All rights reserved.
Author Address [Diedhiou, Calliste J.; Popova, Olga V.; Golldack, Dortje] Univ Bielefeld, Fac Biol, Dept Physiol & Biochem Plants, D-33615 Bielefeld, Germany. Golldack, D (reprint author), Univ Bielefeld, Fac Biol, Dept Physiol & Biochem Plants, D-33615 Bielefeld, Germany. dortje.golldack@uni-bielefeld.de
ISSN 0176-1617
ISBN 0176-1617
29-Character Source Abbreviation J. Plant Physiol.
Year Published 2009
Volume 166
Issue 7
Beginning Page 697-711
Digital Object Identifier (DOI) 10.1016/j.jplph.2008.09.015
Unique Article Identifier WOS:000265461500003
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