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Publication Type J
Authors Debez, A., H. P. Braun, A. Pich, W. Taamalli, H. W. Koyro, C. Abdelly and B. Huchzermeyer
Title Proteomic and physiological responses of the halophyte Cakile maritima to moderate salinity at the germinative and vegetative stages
Source Journal of Proteomics
Author Keywords Germination Halophytes Leaves Proteome Salt-tolerance rice oryza-sativa arabidopsis seed-germination salt stress chloroplast preparations endoplasmic-reticulum reserve mobilization salicornia-europaea extreme halophyte oil mobilization wheat-varieties
Abstract Responses of the halophyte Cakile maritima to moderate salinity were addressed at germination and vegetative stages by bringing together proteomics and eco-physiological approaches. 75 mM NaCl-salinity delayed significantly the germination process and decreased slightly the seed germination percentage compared to salt-free conditions. Monitoring the proteome profile between 0 h and 120 h after seed sowing revealed a delay in the degradation of seed storage proteins when germination took place under salinity, which may explain the slower germination rate observed. Of the sixty-seven proteins identified by mass spectrometry, several proteins involved in glycolysis, amino acid metabolism, photosynthesis, and protein folding showed significantly increased abundance during germination. This pattern was less pronounced under salinity. At the vegetative stage, 100 mM NaCl-salinity stimulated significantly the plant growth, which was sustained by enhanced leaf expansion, water content, and photosynthetic activity. comparative proteome analyses of leaf tissue revealed 44 proteins with different abundance changes, most of which being involved in energy metabolism. A specific set of proteins predominantly involved in photosynthesis and respiration showed significantly higher abundance in salt-treated plants. Altogether, combining proteomics with eco-physiological tools provides valuable information, which contributes to improve our understanding in the salt-response of this halophyte during its life cycle. (C) 2012 Elsevier B.V. All rights reserved.
Author Address [Debez, Ahmed; Taamalli, Wael; Abdelly, Chedly] Ctr Biotechnol Technopole Borj Cedria CBBC, LPE, Hammam Lif 2050, Tunisia. [Debez, Ahmed; Huchzermeyer, Bernhard] Leibniz Univ Hannover, Inst Bot, D-30419 Hannover, Germany. [Braun, Hans-Peter] Leibniz Univ Hannover, Inst Pflanzengenet, Dept Plant Prote 5, D-30419 Hannover, Germany. [Pich, Andreas] Hannover Med Sch, Inst Toxicol, D-30625 Hannover, Germany. [Koyro, Hans-Werner] Univ Giessen, Inst Pflanzenokol, D-35392 Giessen, Germany. Debez, A (reprint author), Ctr Biotechnol Technopole Borj Cedria CBBC, LPE, BP 901, Hammam Lif 2050, Tunisia. ahmed.debez@cbbc.mrt.tn
ISSN 1874-3919
ISBN 1874-3919
29-Character Source Abbreviation J. Proteomics
Publication Date Oct
Year Published 2012
Volume 75
Issue 18
Beginning Page 5667-5694
Digital Object Identifier (DOI) 10.1016/j.jprot.2012.08.012
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