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Publication Type J
Authors Ben Hsouna, A., M. Hfaiedh, S. Ben Slima, W. Ben Romdhane, B. Ben Akacha, M. T. Bouterra, W. Dhifi, W. Mnif, F. Brini, R. Ben Saad and R. Ben Salah
Title Antioxidant and hepatoprotective effects of novel heteropolysaccharide isolated from Lobularia maritima on CCl4-induced liver injury in rats
Source Food Science & Nutrition
Language English
Author Keywords CCl4 genotoxicity halophyte Lobularia maritima hepatoprotective oxidative stress polysaccharides in-vitro extracellular polysaccharides chemical-composition bioactive compounds free-radicals leaf extract purification mechanisms fibrosis ginseng Food Science & Technology
Abstract The aim of the present study was to investigate the extraction and the characterization of a novel heteropolysaccharide from Tunisian halophyte Lobularia maritima (LmPS). We were also interested in its antioxidant, anti-inflammatory, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced liver injury in rats. LmPS physicochemical properties were evaluated by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), thermogravimetric analysis (TGA), and UV absorption. According to TLC and HPLC results, LmPS was a heteropolysaccharide composed of glucose, galactose, and xylose. Its molecular weight was 130.62 kDa. This heteropolysaccharide was characterized by a significant antioxidant potential and was efficient against oxidative stress and CCL4-induced hepatotoxicity in rat Wistar models (n = 8) treated with a single dose of LmPS 250 mg/kg of body weight. This was evidenced by a significant increase in serum marker enzymes specially aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH). The cytokines released after stimulation of rats with LmPS showed high anti-inflammatory profiles with an increased rate of interleukine-10 (IL-10) with 0.03 pg/mL compared to animals treated only with CCl4. On the contrary, we noticed a decrease of the other cytokines (tumor necrosis factor (alpha: TNF-alpha, interleukine-6: IL-6, transforming growth factor beta 1: TGF-beta 1) with average concentration values of <0.2, 0.1, and 0.04 pg/mL, respectively. Besides, histopathological examinations revealed that CCl4 causes acute liver damage, characterized by extensive hepatocellular necrosis, vacuolization, and inflammatory cell infiltration, as well as DNA fragmentation. LmPS administration at a dose of 250 mg/kg resulted in a significant hepatoprotection, evidenced by a reduction of CCl4-induced oxidative damage for all tested markers. These findings eagerly confirmed that LmPS was effective in the protection against CCl4-induced hepatotoxicity and genotoxicity. It, therefore, suggested a potential therapeutic use of this polysaccharide as an alternative medicine for patients with acute liver diseases.
Author Address [Ben Hsouna, Anis] Fac Sci Gafsa, Dept Life Sci, Zarroug 2112, Gafsa, Tunisia. [Ben Hsouna, Anis; Ben Romdhane, Walid; Ben Akacha, Boutheina; Bouterra, Mohamed Taieb; Brini, Faical; Ben Saad, Rania] Ctr Biotechnol Sfax, Lab Biotechnol & Plant Improvement, Sfax, Tunisia. [Hfaiedh, Mbarka] Univ Gabes, Higher Inst Appl Biol Medenine, Res Unit Act Biomol Valorisat, Medenine, Tunisia. [Ben Slima, Sirine; Ben Salah, Riadh] Ctr Biotechnol Sfax, Lab Microorganisms & Biomol LMB, Sfax 3018, Tunisia. [Dhifi, Wissal] Univ Manouba, Higher Inst Biotechnol Sidi Thabet, Lab Biotechnol & Valorisat BioGeoRessources, Ariana, Tunisia. [Mnif, Wissem] Univ Bisha, Fac Sci & Arts Balgarn, Dept Chem, POB 199, Bisha 61922, Saudi Arabia. [Mnif, Wissem] Univ Manouba, ISBST, Biotechpole Sidi Thabet, BVBGR LR11ES31, Ariana, Tunisia. Ben Hsouna, A (corresponding author), Fac Sci Gafsa, Dept Life Sci, Zarroug 2112, Gafsa, Tunisia.; Ben Salah, R (corresponding author), Ctr Biotechnol Sfax, Lab Microorganisms & Biomol LMB, Sfax 3018, Tunisia.; Mnif, W (corresponding author), Univ Bisha, Fac Sci & Arts Balgarn, Dept Chem, POB 199, Bisha 61922, Saudi Arabia. benhsounanis@yahoo.fr; w_mnif@yahoo.fr; riadh-fss@yahoo.fr
ISSN 2048-7177
ISBN 2048-7177
29-Character Source Abbreviation Food Sci. Nutr.
Beginning Page 14
Digital Object Identifier (DOI) 10.1002/fsn3.2836
Unique Article Identifier WOS:000777547300001
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