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Publication Type J
Authors Baysal, I., M. Ekizoglu, A. Ertas, B. Temiz, H. G. Agalar, S. Yabanoglu-Ciftci, H. Temel, G. Ucar and F. P. Turkmenoglu
Title Identification of Phenolic Compounds by LC-MS/MS and Evaluation of Bioactive Properties of Two Edible Halophytes: Limonium effusum and L. sinuatum
Source Molecules
Language English
Author Keywords Limonium effusum Limonium sinuatum Plumbaginaceae phenolics LC-MS MS antioxidant antimicrobial anticholinesterase antityrosinase pancreatic lipase enzyme inhibition antimicrobial activities inhibitory-activity antioxidant activities pancreatic lipase tyrosinase extracts profile plants kuntze root Biochemistry & Molecular Biology Chemistry
Abstract This work aimed to evaluate the phenolic content and in vitro antioxidant, antimicrobial and enzyme inhibitory activities of the methanol extracts and their fractions of two edible halophytic Limonium species, L. effusum (LE) and L. sinuatum (LS). The total phenolic content resulted about two-fold higher in the ethyl acetate fraction of LE (522.82 +/- 5.67 mg GAE/g extract) than in that of LS (274.87 +/- 1.87 mg GAE/g extract). LC-MS/MS analysis indicated that tannic acid was the most abundant phenolic acid in both species (71,439.56 +/- 3643.3 mu g/g extract in LE and 105,453.5 +/- 5328.1 mu g/g extract in LS), whereas hyperoside was the most abundant flavonoid (14,006.90 +/- 686.1 mu g/g extract in LE and 1708.51 +/- 83.6 mu g/g extract in LS). The antioxidant capacity was evaluated by DPPH and TAC assays, and the stronger antioxidant activity in ethyl acetate fractions was highlighted. Both species were more active against Gram-positive bacteria than Gram negatives and showed considerable growth inhibitions against tested fungi. Interestingly, selective acetylcholinesterase (AChE) activity was observed with LE and LS. Particularly, the water fraction of LS strongly inhibited AChE (IC50 = 0.199 +/- 0.009 mu g/mL). The ethyl acetate fractions of LE and LS, as well as the n-hexane fraction of LE, exhibited significant antityrosinase activity (IC50 = 245.56 +/- 3.6, 295.18 +/- 10.57 and 148.27 +/- 3.33 mu g/mL, respectively). The ethyl acetate fraction and methanol extract of LS also significantly inhibited pancreatic lipase (IC50 = 83.76 +/- 4.19 and 162.2 +/- 7.29 mu g/mL, respectively). Taken together, these findings warrant further investigations to assess the potential of LE and LS as a bioactive source that can be exploited in pharmaceutical, cosmetics and food industries.
Author Address [Baysal, Ipek] Hacettepe Univ, Vocat Sch Hlth Serv, TR-06100 Ankara, Turkey. [Ekizoglu, Melike] Hacettepe Univ, Fac Pharm, Dept Pharmaceut Microbiol, TR-06100 Ankara, Turkey. [Ertas, Abdulselam] Dicle Univ, Fac Pharm, Dept Pharmacognosy, TR-21280 Diyarbakir, Turkey. [Temiz, Burak; Agalar, Hale Gamze] Anadolu Univ, Fac Pharm, Dept Pharmacognosy, TR-26470 Eskisehir, Turkey. [Yabanoglu-Ciftci, Samiye; Ucar, Gulberk] Hacettepe Univ, Fac Pharm, Dept Biochem, TR-06100 Ankara, Turkey. [Temel, Hamdi] Dicle Univ, Fac Pharm, Dept Pharmaceut Chem, TR-21280 Diyarbakir, Turkey. [Turkmenoglu, Fatma Pinar] Anadolu Univ, Fac Pharm, Dept Pharmaceut Bot, TR-26470 Eskisehir, Turkey. Turkmenoglu, FP (corresponding author), Anadolu Univ, Fac Pharm, Dept Pharmaceut Bot, TR-26470 Eskisehir, Turkey. ipekbaysal@hacettepe.edu.tr; melike@hacettepe.edu.tr; abdulselam.ertas@dicle.edu.tr; burak_temiz@anadolu.edu.tr; hgduymus@anadolu.edu.tr; samiye@hacettepe.edu.tr; htemel@dicle.edu.tr; gulberk@hacettepe.edu.tr; fatmapinarkafa@anadolu.edu.tr
29-Character Source Abbreviation Molecules
Publication Date Jul
Year Published 2021
Volume 26
Issue 13
Beginning Page 18
Digital Object Identifier (DOI) 10.3390/molecules26134040
Unique Article Identifier WOS:000670978500001

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