Loading content, please wait..
loading..
Logo
Version 3.22
or
Publication Type J
Authors Gao, L., J. B. Ma, Y. H. Liu, Y. Huang, O. A. A. Mohamad, H. C. Jiang, D. Egamberdieva, W. J. Li and L. Li
Title Diversity and Biocontrol Potential of Cultivable Endophytic Bacteria Associated with Halophytes from the West Aral Sea Basin
Source Microorganisms
Language English
Author Keywords endophytes halophytes diversity antifungal activity Aral Sea plant-growth nov. Microbiology
Abstract Endophytes associated with halophytes may contribute to the host's adaptation to adverse environmental conditions through improving their stress tolerance and protecting them from various soil-borne pathogens. In this study, the diversity and antifungal activity of endophytic bacteria associated with halophytic samples growing on the shore of the western Aral Sea in Uzbekistan were investigated. The endophytic bacteria were isolated from the nine halophytic samples by using the culture-dependent method and identified according to their 16S rRNA gene sequences. The screening of endophytic bacterial isolates with the ability to inhibit pathogenic fungi was completed by the plate confrontation method. A total of 289 endophytic bacterial isolates were isolated from the nine halophytes, and they belong to Firmicutes, Actinobacteria, and Proteobacteria. The predominant genera of the isolated endophytic bacteria were Bacillus, Staphylococcus, and Streptomyces, accounting for 38.5%, 24.7%, and 12.5% of the total number of isolates, respectively. The comparative analysis indicated that the isolation effect was better for the sample S8, with the highest diversity and richness indices. The diversity index of the sample S7 was the lowest, while the richness index of samples S5 and S6 was the lowest. By comparing the isolation effect of 12 different media, it was found that the M7 medium had the best performance for isolating endophytic bacteria associated with halophytes in the western Aral Sea Basin. In addition, the results showed that only a few isolates have the ability to produce ex-enzymes, and eight and four endophytic bacterial isolates exhibited significant inhibition to the growth of Valsa mali and Verticillium dahlia, respectively. The results of this study indicated that halophytes are an important source for the selection of microbes that may protect plant from soil-borne pathogens.
Author Address [Gao, Lei; Ma, Jinbiao; Liu, Yonghong; Huang, Yin; Mohamad, Osama Abdalla Abdelshafy; Jiang, Hongchen; Li, Wenjun; Li, Li] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China. [Gao, Lei] Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China. [Jiang, Hongchen] China Univ Geosci, State Key Lab Biogeol & Environm Geol, Wuhan 430074, Peoples R China. [Egamberdieva, Dilfuza] Natl Univ Uzbekistan, Fac Biol, Tashkent 100174, Uzbekistan. [Egamberdieva, Dilfuza] Leibniz Ctr Agr Landscape Res ZALF, D-15374 Muncheberg, Germany. [Li, Wenjun] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangdong Prov Key Lab Plant Resources, Guangzhou 510275, Guangdong, Peoples R China. Li, WJ; Li, L (corresponding author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China.; Li, WJ (corresponding author), Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangdong Prov Key Lab Plant Resources, Guangzhou 510275, Guangdong, Peoples R China. gaolei19@mails.ucas.ac.cn; majinbiao@ms.xjb.ac.cn; liuyh@ms.xjb.ac.cn; yinhuang0929@163.com; osama@aru.edu.eg; jiangh@cug.edu.cn; egamberdieva@yahoo.com; liact@hotmail.com; lili.bobo@outlook.com
29-Character Source Abbreviation Microorganisms
Publication Date Jul
Year Published 2021
Volume 9
Issue 7
Beginning Page 16
Digital Object Identifier (DOI) 10.3390/microorganisms9071448
Unique Article Identifier WOS:000676229400001

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed