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Authors Yin, HJ; Li, MZ; Li, DD; Khan, SA; Hepworth, SR; Wang, SM
Author Full Name Yin, Hongju; Li, Mengzhan; Li, Dingding; Khan, Sardar-Ali; Hepworth, Shelley R.; Wang, Suo-Min
Title Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte
Source BMC PLANT BIOLOGY
Language English
Document Type Article
Author Keywords RNA-sequencing; Osmotic stress; Salt; Protein kinases; Transcription factors; Proteolysis
Keywords Plus ABIOTIC STRESS TOLERANCE; PROTEIN-KINASE; ABSCISIC-ACID; AGRICULTURAL BIOTECHNOLOGY; ZYGOPHYLLUM-XANTHOXYLUM; DROUGHT TOLERANCE; CROP IMPROVEMENT; GENE-EXPRESSION; IN-VITRO; ABA
Abstract Background: Zygophyllum xanthoxylum is a succulent xerophyte with remarkable tolerance to diverse abiotic stresses. Previous studies have revealed important physiological mechanisms and identified functional genes associated with stress tolerance. However, knowledge of the regulatory genes conferring stress tolerance in this species is poorly understood. Results: Here, we present a comprehensive analysis of regulatory genes based on the transcriptome of Z. xanthoxylum roots exposed to osmotic stress and salt treatments. Significant changes were observed in transcripts related to known and obscure stress-related hormone signaling pathways, in particular abscisic acid and auxin. Significant changes were also found among key classes of early response regulatory genes encoding protein kinases, transcription factors, and ubiquitin-mediated proteolysis machinery. Network analysis shows a highly integrated matrix formed by these conserved and novel gene products associated with osmotic stress and salt in Z. xanthoxylum. Among them, two previously uncharacterized NAC (NAM/ATAF/CUC) transcription factor genes, ZxNAC083 (Unigene16368_All) and ZxNAC035 (CL6534.Contig1_All), conferred tolerance to salt and drought stress when constitutively overexpressed in Arabidopsis plants. Conclusions: This study provides a unique framework for understanding osmotic stress and salt adaptation in Z. xanthoxylum including novel gene targets for engineering stress tolerance in susceptible crop species.
Author Address [Yin, Hongju; Li, Mengzhan; Li, Dingding; Khan, Sardar-Ali; Hepworth, Shelley R.; Wang, Suo-Min] Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China; [Yin, Hongju; Li, Mengzhan; Li, Dingding; Khan, Sardar-Ali; Hepworth, Shelley R.; Wang, Suo-Min] Lanzhou Univ, Minist Agr & Rural Affairs, Key Lab Grassland Livestock Ind Innovat, Lanzhou 730020, Gansu, Peoples R China; [Yin, Hongju; Li, Mengzhan; Li, Dingding; Khan, Sardar-Ali; Hepworth, Shelley R.; Wang, Suo-Min] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Gansu, Peoples R China; [Hepworth, Shelley R.] Carleton Univ, Inst Biochem, Dept Biol, Ottawa, ON, Canada
Reprint Address Yin, HJ; Wang, SM (corresponding author), Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China.; Yin, HJ; Wang, SM (corresponding author), Lanzhou Univ, Minist Agr & Rural Affairs, Key Lab Grassland Livestock Ind Innovat, Lanzhou 730020, Gansu, Peoples R China.; Yin, HJ; Wang, SM (corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Gansu, Peoples R China.
E-mail Address yinhj@lzu.edu.cn; smwang@lzu.edu.cn
ORCID Number Hepworth, Shelley/0000-0002-6496-3792
Funding Agency and Grant Number National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730093, 31601992]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [862920, 862240]
Funding Text This work has been jointly supported by the National Natural Science Foundation of China (Grant no. 31730093 and 31601992), and the Fundamental Research Funds for the Central Universities (Grant No. 862920 and 862240).
Times Cited 4
Total Times Cited Count (WoS, BCI, and CSCD) 4
Publisher BMC
Publisher City LONDON
Publisher Address CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
ISSN 1471-2229
29-Character Source Abbreviation BMC PLANT BIOL
ISO Source Abbreviation BMC Plant Biol.
Publication Date FEB 28
Year Published 2019
Volume 19
Article Number 88
Digital Object Identifier (DOI) 10.1186/s12870-019-1686-1
Page Count 15
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number HN2TT
Unique Article Identifier WOS:000460038500002
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