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| Authors | Wu, GQ; Xi, JJ; Wang, Q; Bao, AK; Ma, Q; Zhang, JL; Wang, SM |
| Author Full Name | Wu, Guo-Qiang; Xi, Jie-Jun; Wang, Qian; Bao, Ai-Ke; Ma, Qing; Zhang, Jin-Lin; Wang, Suo-Min |
| Title | The ZxNHX gene encoding tonoplast Na+/H+ antiporter from the xerophyte Zygophyllum xanthoxylum plays important roles in response to salt and drought |
| Source | JOURNAL OF PLANT PHYSIOLOGY |
| Language | English |
| Document Type | Article |
| Author Keywords | Zygophyllum xanthoxylum; Tonoplast Na+/H+ antiporter; Sodium accumulation; Salt and drought tolerance |
| Keywords Plus | ARABIDOPSIS-THALIANA; MOLECULAR CHARACTERIZATION; RESISTANT PLANTS; EXPRESSION; TOLERANCE; STRESS; K+; OVEREXPRESSION; ACCUMULATION; TRANSPORT |
| Abstract | Sodium (Na+) has been found to play important roles in the adaptation of xerophytic species to drought conditions. The tonoplast Na+/H+ antiporter (NHX) proved to be involved in the compartmentalization of Na+ into vacuoles from the cytosol. In this study, a gene (ZxNHX) encoding tonoplast Na+/H+ antiporter was isolated and characterized in Zygophyllum xanthoxylum, a succulent xerophyte growing in desert areas of northwest China. The results revealed that ZxNHX consisted of 532 amino acid residues with a conserved binding domain ((LFFIYLLPPI87)-L-78) for amiloride and shared high similarity (73-81%) with the identified tonoplast Na+/H+ antiporters in other plant species. Semi-quantitative RT-PCR analysis showed that the mRNA level of ZxNHX was significantly higher in the leaf than in stem or root. The transcript abundance of ZxNHX in 1 xanthoxylum subjected to salt (5-150 mM NaCl) or drought (50-15% of field water capacity (FWC)) was 1.4-8.4 times or 2.3-4.4 times that of plants grown in the absence of NaCl or 70% of FWC, respectively. Leaf Na+ concentration in plants exposed to salt or drought was 1.7-5.2 times or 1.5-2.2 times that of corresponding control plants, respectively. It is clear that there is a positive correlation between up-regulation of ZxNHX and accumulation of Na+ in Z. xanthoxylum exposed to salt or drought. Furthermore. Z. xanthoxylum accumulated larger amounts of Na+ than K+ in the leaf under drought conditions, even in low salt soil. In summary, our results suggest that ZxNHX encodes a tonoplast Na+/H+ antiporter and plays important roles in Na+ accumulation and homeostasis of Z. xanthoxylum under salt and drought conditions. (C) 2010 Elsevier GmbH. All rights reserved. |
| Author Address | [Wu, Guo-Qiang; Xi, Jie-Jun; Wang, Qian; Bao, Ai-Ke; Ma, Qing; Zhang, Jin-Lin; Wang, Suo-Min] Lanzhou Univ, Minist Agr, Sch Pastoral Agr Sci & Technol, Key Lab Grassland Agroecosyst, Lanzhou 730000, Peoples R China |
| Reprint Address | Wang, SM (corresponding author), Lanzhou Univ, Minist Agr, Sch Pastoral Agr Sci & Technol, Key Lab Grassland Agroecosyst, POB 61, Lanzhou 730000, Peoples R China. |
| E-mail Address | smwang@lzu.edu.cn |
| ResearcherID Number | Zhang, Jinlin/AAQ-6331-2020 |
| ORCID Number | Zhang, Jinlin/0000-0002-3562-920X |
| Funding Agency and Grant Number | National Basic Research Program of ChinaNational Basic Research Program of China [2007CB108901]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30770347, 31072073] |
| Funding Text | This work was supported by the National Basic Research Program of China (973 program, grant no. 2007CB108901) and the National Natural Science Foundation of China (grant nos. 30770347 and 31072073). The authors wish to thank anonymous reviewers for their comments on the initial version of the manuscript. We also thank Roger. L. Davies, New Zealand China Friendship Society (Gansu Projects Committee), for his assistance in revising the manuscript. |
| Times Cited | 58 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 85 |
| Publisher | ELSEVIER GMBH |
| Publisher City | MUNICH |
| Publisher Address | HACKERBRUCKE 6, 80335 MUNICH, GERMANY |
| ISSN | 0176-1617 |
| 29-Character Source Abbreviation | J PLANT PHYSIOL |
| ISO Source Abbreviation | J. Plant Physiol. |
| Publication Date | MAY 15 |
| Year Published | 2011 |
| Volume | 168 |
| Issue | 8 |
| Beginning Page | 758 |
| Ending Page | 767 |
| Digital Object Identifier (DOI) | 10.1016/j.jplph.2010.10.015 |
| Page Count | 10 |
| Web of Science Category | Plant Sciences |
| Subject Category | Plant Sciences |
| Document Delivery Number | 767UW |
| Unique Article Identifier | WOS:000290884800003 |
| Plants associated with this reference |