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Authors Wu, GQ; Xi, JJ; Wang, Q; Bao, AK; Ma, Q; Zhang, JL; Wang, SM
Author Full Name Wu, Guo-Qiang; Xi, Jie-Jun; Wang, Qian; Bao, Ai-Ke; Ma, Qing; Zhang, Jin-Lin; Wang, Suo-Min
Title The ZxNHX gene encoding tonoplast Na+/H+ antiporter from the xerophyte Zygophyllum xanthoxylum plays important roles in response to salt and drought
Source JOURNAL OF PLANT PHYSIOLOGY
Language English
Document Type Article
Author Keywords Zygophyllum xanthoxylum; Tonoplast Na+/H+ antiporter; Sodium accumulation; Salt and drought tolerance
Keywords Plus ARABIDOPSIS-THALIANA; MOLECULAR CHARACTERIZATION; RESISTANT PLANTS; EXPRESSION; TOLERANCE; STRESS; K+; OVEREXPRESSION; ACCUMULATION; TRANSPORT
Abstract Sodium (Na+) has been found to play important roles in the adaptation of xerophytic species to drought conditions. The tonoplast Na+/H+ antiporter (NHX) proved to be involved in the compartmentalization of Na+ into vacuoles from the cytosol. In this study, a gene (ZxNHX) encoding tonoplast Na+/H+ antiporter was isolated and characterized in Zygophyllum xanthoxylum, a succulent xerophyte growing in desert areas of northwest China. The results revealed that ZxNHX consisted of 532 amino acid residues with a conserved binding domain ((LFFIYLLPPI87)-L-78) for amiloride and shared high similarity (73-81%) with the identified tonoplast Na+/H+ antiporters in other plant species. Semi-quantitative RT-PCR analysis showed that the mRNA level of ZxNHX was significantly higher in the leaf than in stem or root. The transcript abundance of ZxNHX in 1 xanthoxylum subjected to salt (5-150 mM NaCl) or drought (50-15% of field water capacity (FWC)) was 1.4-8.4 times or 2.3-4.4 times that of plants grown in the absence of NaCl or 70% of FWC, respectively. Leaf Na+ concentration in plants exposed to salt or drought was 1.7-5.2 times or 1.5-2.2 times that of corresponding control plants, respectively. It is clear that there is a positive correlation between up-regulation of ZxNHX and accumulation of Na+ in Z. xanthoxylum exposed to salt or drought. Furthermore. Z. xanthoxylum accumulated larger amounts of Na+ than K+ in the leaf under drought conditions, even in low salt soil. In summary, our results suggest that ZxNHX encodes a tonoplast Na+/H+ antiporter and plays important roles in Na+ accumulation and homeostasis of Z. xanthoxylum under salt and drought conditions. (C) 2010 Elsevier GmbH. All rights reserved.
Author Address [Wu, Guo-Qiang; Xi, Jie-Jun; Wang, Qian; Bao, Ai-Ke; Ma, Qing; Zhang, Jin-Lin; Wang, Suo-Min] Lanzhou Univ, Minist Agr, Sch Pastoral Agr Sci & Technol, Key Lab Grassland Agroecosyst, Lanzhou 730000, Peoples R China
Reprint Address Wang, SM (corresponding author), Lanzhou Univ, Minist Agr, Sch Pastoral Agr Sci & Technol, Key Lab Grassland Agroecosyst, POB 61, Lanzhou 730000, Peoples R China.
E-mail Address smwang@lzu.edu.cn
ResearcherID Number Zhang, Jinlin/AAQ-6331-2020
ORCID Number Zhang, Jinlin/0000-0002-3562-920X
Funding Agency and Grant Number National Basic Research Program of ChinaNational Basic Research Program of China [2007CB108901]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30770347, 31072073]
Funding Text This work was supported by the National Basic Research Program of China (973 program, grant no. 2007CB108901) and the National Natural Science Foundation of China (grant nos. 30770347 and 31072073). The authors wish to thank anonymous reviewers for their comments on the initial version of the manuscript. We also thank Roger. L. Davies, New Zealand China Friendship Society (Gansu Projects Committee), for his assistance in revising the manuscript.
Times Cited 58
Total Times Cited Count (WoS, BCI, and CSCD) 85
Publisher ELSEVIER GMBH
Publisher City MUNICH
Publisher Address HACKERBRUCKE 6, 80335 MUNICH, GERMANY
ISSN 0176-1617
29-Character Source Abbreviation J PLANT PHYSIOL
ISO Source Abbreviation J. Plant Physiol.
Publication Date MAY 15
Year Published 2011
Volume 168
Issue 8
Beginning Page 758
Ending Page 767
Digital Object Identifier (DOI) 10.1016/j.jplph.2010.10.015
Page Count 10
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number 767UW
Unique Article Identifier WOS:000290884800003
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