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Authors Gao, TG; Ma, CM; Yuan, HJ; Liu, HS; Ma, Q; Flowers, TJ; Wang, SM
Author Full Name Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Flowers, Timothy J.; Wang, Suo-Min
Title ZxNHX1 indirectly participates in controlling K+ homeostasis in the xerophyte Zygophyllum xanthoxylum
Source FUNCTIONAL PLANT BIOLOGY
Language English
Document Type Article
Author Keywords K+ transport; Na+ transport; osmotic stress; succulent; xerophyte; ZxNHX1; Zygophyllum xanthoxylum
Keywords Plus WATER-USE EFFICIENCY; PLASMA-MEMBRANE; SALT-TOLERANCE; POTASSIUM-TRANSPORT; NA+/H+ ANTIPORT; STRESS; GROWTH; PLANTS; ACCUMULATION; EXPRESSION
Abstract The succulent xerophyte Zygophyllum xanthoxylum (Bunge) Engl. can absorb Na+ from the soil as an osmoticum in order to resist osmotic stress. The tonoplast Na+/H+ antiporter ZxNHX1 is essential for maintaining the salt-accumulation characteristics of Z. xanthoxylum by compartmentalizing Na+ into vacuoles. Previous results revealed that the silencing of ZxNHX1 greatly decreased Na+ accumulation in Z. xanthoxylum under 50 mM NaCl due to the weakened compartmentalisation; in addition, K+ concentration also significantly reduced in ZxNHX1-RNAi lines. Yet, whether the reduction of K+ concentration was directly triggered by the silencing of ZxNHX1 remains unclear. In this study, the growth parameters and expression levels of ZxSOS1, ZxHKT1;1, ZxAKT1 and ZxSKOR were measured in wild-type and ZxNHX1-RNAi lines under control or -0.5 MPa osmotic stress. The results showed that the silencing of ZxNHX1 inhibited the plant growth, decreased Na+ concentration in leaves, reduced the transcript abundance of ZxSOS1 and dramatically increased that of ZxHKT1;1 in roots of Z. xanthoxylum under osmotic stress; whereas tissue K+ concentrations and the expression level of ZxSKOR displayed no significant variations, and the expression of ZxAKT1 were significantly reduced in ZxNHX1-RNAi lines under osmotic stress, compared with the wild type. These results suggest that in Z. xanthoxylum, ZxNHX1 can maintain the normal growth by compartmentalizing Na+ into vacuoles, and regulate the spatial distribution of Na+ indirectly by affecting the expressions of ZxSOS1 and ZxHKT1;1. Moreover, the silencing of ZxNHX1 is not the main reason that led to the reduction of K+ concentration in ZxNHX1-RNAi lines under 50 mM NaCl, and ZxNHX1 might be indirectly involved in regulating K+ homeostasis.
Author Address [Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Wang, Suo-Min] Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Peoples R China; [Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Wang, Suo-Min] Lanzhou Univ, Minist Agr & Rural Affairs, Key Lab Grassland Livestock Ind Innovat, Lanzhou 730020, Peoples R China; [Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Wang, Suo-Min] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Peoples R China; [Yuan, Hui-Jun] Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; [Flowers, Timothy J.] Univ Sussex, Sch Life Sci, Dept Evolut Behav & Environm, Brighton BN1 9QG, Sussex, England
Reprint Address Wang, SM (corresponding author), Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Peoples R China.; Wang, SM (corresponding author), Lanzhou Univ, Minist Agr & Rural Affairs, Key Lab Grassland Livestock Ind Innovat, Lanzhou 730020, Peoples R China.; Wang, SM (corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Peoples R China.
E-mail Address smwang@lzu.edu
ORCID Number Flowers, Timothy/0000-0002-2712-9504
Funding Agency and Grant Number National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730093, 31971405]
Funding Text This work was supported by the National Natural Science Foundation of China (31730093 and 31971405).
Publisher CSIRO PUBLISHING
Publisher City CLAYTON
Publisher Address UNIPARK, BLDG 1, LEVEL 1, 195 WELLINGTON RD, LOCKED BAG 10, CLAYTON, VIC 3168, AUSTRALIA
ISSN 1445-4408
29-Character Source Abbreviation FUNCT PLANT BIOL
ISO Source Abbreviation Funct. Plant Biol.
Year Published 2021
Volume 48
Issue 4
Beginning Page 402
Ending Page 410
Digital Object Identifier (DOI) 10.1071/FP20185
Page Count 9
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number QT0HB
Unique Article Identifier WOS:000596034300001
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