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| Authors | Gao, TG; Ma, CM; Yuan, HJ; Liu, HS; Ma, Q; Flowers, TJ; Wang, SM |
| Author Full Name | Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Flowers, Timothy J.; Wang, Suo-Min |
| Title | ZxNHX1 indirectly participates in controlling K+ homeostasis in the xerophyte Zygophyllum xanthoxylum |
| Source | FUNCTIONAL PLANT BIOLOGY |
| Language | English |
| Document Type | Article |
| Author Keywords | K+ transport; Na+ transport; osmotic stress; succulent; xerophyte; ZxNHX1; Zygophyllum xanthoxylum |
| Keywords Plus | WATER-USE EFFICIENCY; PLASMA-MEMBRANE; SALT-TOLERANCE; POTASSIUM-TRANSPORT; NA+/H+ ANTIPORT; STRESS; GROWTH; PLANTS; ACCUMULATION; EXPRESSION |
| Abstract | The succulent xerophyte Zygophyllum xanthoxylum (Bunge) Engl. can absorb Na+ from the soil as an osmoticum in order to resist osmotic stress. The tonoplast Na+/H+ antiporter ZxNHX1 is essential for maintaining the salt-accumulation characteristics of Z. xanthoxylum by compartmentalizing Na+ into vacuoles. Previous results revealed that the silencing of ZxNHX1 greatly decreased Na+ accumulation in Z. xanthoxylum under 50 mM NaCl due to the weakened compartmentalisation; in addition, K+ concentration also significantly reduced in ZxNHX1-RNAi lines. Yet, whether the reduction of K+ concentration was directly triggered by the silencing of ZxNHX1 remains unclear. In this study, the growth parameters and expression levels of ZxSOS1, ZxHKT1;1, ZxAKT1 and ZxSKOR were measured in wild-type and ZxNHX1-RNAi lines under control or -0.5 MPa osmotic stress. The results showed that the silencing of ZxNHX1 inhibited the plant growth, decreased Na+ concentration in leaves, reduced the transcript abundance of ZxSOS1 and dramatically increased that of ZxHKT1;1 in roots of Z. xanthoxylum under osmotic stress; whereas tissue K+ concentrations and the expression level of ZxSKOR displayed no significant variations, and the expression of ZxAKT1 were significantly reduced in ZxNHX1-RNAi lines under osmotic stress, compared with the wild type. These results suggest that in Z. xanthoxylum, ZxNHX1 can maintain the normal growth by compartmentalizing Na+ into vacuoles, and regulate the spatial distribution of Na+ indirectly by affecting the expressions of ZxSOS1 and ZxHKT1;1. Moreover, the silencing of ZxNHX1 is not the main reason that led to the reduction of K+ concentration in ZxNHX1-RNAi lines under 50 mM NaCl, and ZxNHX1 might be indirectly involved in regulating K+ homeostasis. |
| Author Address | [Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Wang, Suo-Min] Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Peoples R China; [Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Wang, Suo-Min] Lanzhou Univ, Minist Agr & Rural Affairs, Key Lab Grassland Livestock Ind Innovat, Lanzhou 730020, Peoples R China; [Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Wang, Suo-Min] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Peoples R China; [Yuan, Hui-Jun] Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; [Flowers, Timothy J.] Univ Sussex, Sch Life Sci, Dept Evolut Behav & Environm, Brighton BN1 9QG, Sussex, England |
| Reprint Address | Wang, SM (corresponding author), Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Peoples R China.; Wang, SM (corresponding author), Lanzhou Univ, Minist Agr & Rural Affairs, Key Lab Grassland Livestock Ind Innovat, Lanzhou 730020, Peoples R China.; Wang, SM (corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Peoples R China. |
| E-mail Address | smwang@lzu.edu |
| ORCID Number | Flowers, Timothy/0000-0002-2712-9504 |
| Funding Agency and Grant Number | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730093, 31971405] |
| Funding Text | This work was supported by the National Natural Science Foundation of China (31730093 and 31971405). |
| Publisher | CSIRO PUBLISHING |
| Publisher City | CLAYTON |
| Publisher Address | UNIPARK, BLDG 1, LEVEL 1, 195 WELLINGTON RD, LOCKED BAG 10, CLAYTON, VIC 3168, AUSTRALIA |
| ISSN | 1445-4408 |
| 29-Character Source Abbreviation | FUNCT PLANT BIOL |
| ISO Source Abbreviation | Funct. Plant Biol. |
| Year Published | 2021 |
| Volume | 48 |
| Issue | 4 |
| Beginning Page | 402 |
| Ending Page | 410 |
| Digital Object Identifier (DOI) | 10.1071/FP20185 |
| Page Count | 9 |
| Web of Science Category | Plant Sciences |
| Subject Category | Plant Sciences |
| Document Delivery Number | QT0HB |
| Unique Article Identifier | WOS:000596034300001 |
| Plants associated with this reference |