Authors |
Gao, TG; Ma, CM; Yuan, HJ; Liu, HS; Ma, Q; Flowers, TJ; Wang, SM |
Author Full Name |
Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Flowers, Timothy J.; Wang, Suo-Min |
Title |
ZxNHX1 indirectly participates in controlling K+ homeostasis in the xerophyte Zygophyllum xanthoxylum |
Source |
FUNCTIONAL PLANT BIOLOGY |
Language |
English |
Document Type |
Article |
Author Keywords |
K+ transport; Na+ transport; osmotic stress; succulent; xerophyte; ZxNHX1; Zygophyllum xanthoxylum |
Keywords Plus |
WATER-USE EFFICIENCY; PLASMA-MEMBRANE; SALT-TOLERANCE; POTASSIUM-TRANSPORT; NA+/H+ ANTIPORT; STRESS; GROWTH; PLANTS; ACCUMULATION; EXPRESSION |
Abstract |
The succulent xerophyte Zygophyllum xanthoxylum (Bunge) Engl. can absorb Na+ from the soil as an osmoticum in order to resist osmotic stress. The tonoplast Na+/H+ antiporter ZxNHX1 is essential for maintaining the salt-accumulation characteristics of Z. xanthoxylum by compartmentalizing Na+ into vacuoles. Previous results revealed that the silencing of ZxNHX1 greatly decreased Na+ accumulation in Z. xanthoxylum under 50 mM NaCl due to the weakened compartmentalisation; in addition, K+ concentration also significantly reduced in ZxNHX1-RNAi lines. Yet, whether the reduction of K+ concentration was directly triggered by the silencing of ZxNHX1 remains unclear. In this study, the growth parameters and expression levels of ZxSOS1, ZxHKT1;1, ZxAKT1 and ZxSKOR were measured in wild-type and ZxNHX1-RNAi lines under control or -0.5 MPa osmotic stress. The results showed that the silencing of ZxNHX1 inhibited the plant growth, decreased Na+ concentration in leaves, reduced the transcript abundance of ZxSOS1 and dramatically increased that of ZxHKT1;1 in roots of Z. xanthoxylum under osmotic stress; whereas tissue K+ concentrations and the expression level of ZxSKOR displayed no significant variations, and the expression of ZxAKT1 were significantly reduced in ZxNHX1-RNAi lines under osmotic stress, compared with the wild type. These results suggest that in Z. xanthoxylum, ZxNHX1 can maintain the normal growth by compartmentalizing Na+ into vacuoles, and regulate the spatial distribution of Na+ indirectly by affecting the expressions of ZxSOS1 and ZxHKT1;1. Moreover, the silencing of ZxNHX1 is not the main reason that led to the reduction of K+ concentration in ZxNHX1-RNAi lines under 50 mM NaCl, and ZxNHX1 might be indirectly involved in regulating K+ homeostasis. |
Author Address |
[Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Wang, Suo-Min] Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Peoples R China; [Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Wang, Suo-Min] Lanzhou Univ, Minist Agr & Rural Affairs, Key Lab Grassland Livestock Ind Innovat, Lanzhou 730020, Peoples R China; [Gao, Tian-Ge; Ma, Cui-Min; Yuan, Hui-Jun; Liu, Hai-Shuang; Ma, Qing; Wang, Suo-Min] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Peoples R China; [Yuan, Hui-Jun] Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; [Flowers, Timothy J.] Univ Sussex, Sch Life Sci, Dept Evolut Behav & Environm, Brighton BN1 9QG, Sussex, England |
Reprint Address |
Wang, SM (corresponding author), Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Peoples R China.; Wang, SM (corresponding author), Lanzhou Univ, Minist Agr & Rural Affairs, Key Lab Grassland Livestock Ind Innovat, Lanzhou 730020, Peoples R China.; Wang, SM (corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Peoples R China. |
E-mail Address |
smwang@lzu.edu |
ORCID Number |
Flowers, Timothy/0000-0002-2712-9504 |
Funding Agency and Grant Number |
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730093, 31971405] |
Funding Text |
This work was supported by the National Natural Science Foundation of China (31730093 and 31971405). |
Publisher |
CSIRO PUBLISHING |
Publisher City |
CLAYTON |
Publisher Address |
UNIPARK, BLDG 1, LEVEL 1, 195 WELLINGTON RD, LOCKED BAG 10, CLAYTON, VIC 3168, AUSTRALIA |
ISSN |
1445-4408 |
29-Character Source Abbreviation |
FUNCT PLANT BIOL |
ISO Source Abbreviation |
Funct. Plant Biol. |
Year Published |
2021 |
Volume |
48 |
Issue |
4 |
Beginning Page |
402 |
Ending Page |
410 |
Digital Object Identifier (DOI) |
10.1071/FP20185 |
Page Count |
9 |
Web of Science Category |
Plant Sciences |
Subject Category |
Plant Sciences |
Document Delivery Number |
QT0HB |
Unique Article Identifier |
WOS:000596034300001
|
Plants associated with this reference |
|