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| Authors | Chai, WW; Wang, WY; Ma, Q; Yin, HJ; Hepworth, SR; Wang, SM |
| Author Full Name | Chai, Wei-Wei; Wang, Wen-Ying; Ma, Qing; Yin, Hong-Ju; Hepworth, Shelley R.; Wang, Suo-Min |
| Title | Comparative transcriptome analysis reveals unique genetic adaptations conferring salt tolerance in a xerohalophyte |
| Source | FUNCTIONAL PLANT BIOLOGY |
| Language | English |
| Document Type | Article |
| Author Keywords | glycophyte; RNA-seq; salt response; stimulus; xerohalophyte; Zygophyllum xanthoxylum |
| Keywords Plus | STRESS TOLERANCE; GLUTATHIONE TRANSFERASE; ZYGOPHYLLUM-XANTHOXYLUM; ARABIDOPSIS-THALIANA; ABIOTIC STRESS; DROUGHT TOLERANCE; OXIDATIVE STRESS; K+ HOMEOSTASIS; NA+ TRANSPORT; SAUR PROTEINS |
| Abstract | Most studies on salt tolerance in plants have been conducted using glycophytes like Arabidopsis thaliana (L.) Heynh., with limited resistance to salinity. The xerohalophyte Zygophyllum xanthoxylum (Bunge) Engl. is a salt-accumulating desert plant that efficiently transports Na+ into vacuoles to manage salt and exhibits increased growth under salinity conditions, suggesting a unique transcriptional response compared with glycophytes. We used transcriptome profiling by RNA-seq to compare gene expression in roots of Z. xanthoxylum and A. thaliana under 50 mM NaCl treatments. Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analysis suggested that 50 mM NaCl was perceived as a stimulus for Z. xanthoxylum whereas a stress for A. thaliana. Exposure to 50 mM NaCl caused metabolic shifts towards gluconeogenesis to stimulate growth of Z. xanthoxylum, but triggered defensive systems in A. thaliana. Compared with A. thaliana, a vast array of ion transporter genes was induced in Z. xanthoxylum, revealing an active strategy to uptake Na+ and nutrients from the environment. An ascorbate-glutathione scavenging system for reactive oxygen species was also crucial in Z. xanthoxylum, based on high expression of key enzyme genes. Finally, key regulatory genes for the biosynthesis pathways of abscisic acid and gibberellin showed distinct expression patterns between the two species and auxin response genes were more active in Z. xanthoxylum compared with A. thaliana. Our results provide an important framework for understanding unique patterns of gene expression conferring salt resistance in Z. xanthoxylum. |
| Author Address | [Chai, Wei-Wei; Wang, Wen-Ying; Ma, Qing; Yin, Hong-Ju; Hepworth, Shelley R.; Wang, Suo-Min] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China; [Chai, Wei-Wei; Wang, Wen-Ying; Ma, Qing; Yin, Hong-Ju; Hepworth, Shelley R.; Wang, Suo-Min] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Key Lab Grassland Livestock Ind Innovat, Minist Agr & Rural Affairs, Lanzhou 730020, Gansu, Peoples R China; [Hepworth, Shelley R.] Carleton Univ, Inst Biochem, Dept Biol, Ottawa, ON, Canada |
| Reprint Address | Wang, SM (corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China.; Wang, SM (corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Key Lab Grassland Livestock Ind Innovat, Minist Agr & Rural Affairs, Lanzhou 730020, Gansu, Peoples R China. |
| E-mail Address | smwang@lzu.edu.cn |
| ORCID Number | Hepworth, Shelley/0000-0002-6496-3792 |
| Funding Agency and Grant Number | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730093, 31470503]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [lzujbky-2018-k01] |
| Funding Text | This research was supported by the National Natural Science Foundation of China (31730093 and 31470503) and the Fundamental Research Funds for the Central Universities (lzujbky-2018-k01). |
| Times Cited | 4 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 5 |
| Publisher | CSIRO PUBLISHING |
| Publisher City | CLAYTON |
| Publisher Address | UNIPARK, BLDG 1, LEVEL 1, 195 WELLINGTON RD, LOCKED BAG 10, CLAYTON, VIC 3168, AUSTRALIA |
| ISSN | 1445-4408 |
| 29-Character Source Abbreviation | FUNCT PLANT BIOL |
| ISO Source Abbreviation | Funct. Plant Biol. |
| Year Published | 2019 |
| Volume | 46 |
| Issue | 7 |
| Beginning Page | 670 |
| Ending Page | 683 |
| Digital Object Identifier (DOI) | 10.1071/FP18295 |
| Page Count | 14 |
| Web of Science Category | Plant Sciences |
| Subject Category | Plant Sciences |
| Document Delivery Number | IK7TS |
| Unique Article Identifier | WOS:000476795600007 |
| Plants associated with this reference |