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Authors Chai, WW; Wang, WY; Ma, Q; Yin, HJ; Hepworth, SR; Wang, SM
Author Full Name Chai, Wei-Wei; Wang, Wen-Ying; Ma, Qing; Yin, Hong-Ju; Hepworth, Shelley R.; Wang, Suo-Min
Title Comparative transcriptome analysis reveals unique genetic adaptations conferring salt tolerance in a xerohalophyte
Source FUNCTIONAL PLANT BIOLOGY
Language English
Document Type Article
Author Keywords glycophyte; RNA-seq; salt response; stimulus; xerohalophyte; Zygophyllum xanthoxylum
Keywords Plus STRESS TOLERANCE; GLUTATHIONE TRANSFERASE; ZYGOPHYLLUM-XANTHOXYLUM; ARABIDOPSIS-THALIANA; ABIOTIC STRESS; DROUGHT TOLERANCE; OXIDATIVE STRESS; K+ HOMEOSTASIS; NA+ TRANSPORT; SAUR PROTEINS
Abstract Most studies on salt tolerance in plants have been conducted using glycophytes like Arabidopsis thaliana (L.) Heynh., with limited resistance to salinity. The xerohalophyte Zygophyllum xanthoxylum (Bunge) Engl. is a salt-accumulating desert plant that efficiently transports Na+ into vacuoles to manage salt and exhibits increased growth under salinity conditions, suggesting a unique transcriptional response compared with glycophytes. We used transcriptome profiling by RNA-seq to compare gene expression in roots of Z. xanthoxylum and A. thaliana under 50 mM NaCl treatments. Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analysis suggested that 50 mM NaCl was perceived as a stimulus for Z. xanthoxylum whereas a stress for A. thaliana. Exposure to 50 mM NaCl caused metabolic shifts towards gluconeogenesis to stimulate growth of Z. xanthoxylum, but triggered defensive systems in A. thaliana. Compared with A. thaliana, a vast array of ion transporter genes was induced in Z. xanthoxylum, revealing an active strategy to uptake Na+ and nutrients from the environment. An ascorbate-glutathione scavenging system for reactive oxygen species was also crucial in Z. xanthoxylum, based on high expression of key enzyme genes. Finally, key regulatory genes for the biosynthesis pathways of abscisic acid and gibberellin showed distinct expression patterns between the two species and auxin response genes were more active in Z. xanthoxylum compared with A. thaliana. Our results provide an important framework for understanding unique patterns of gene expression conferring salt resistance in Z. xanthoxylum.
Author Address [Chai, Wei-Wei; Wang, Wen-Ying; Ma, Qing; Yin, Hong-Ju; Hepworth, Shelley R.; Wang, Suo-Min] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China; [Chai, Wei-Wei; Wang, Wen-Ying; Ma, Qing; Yin, Hong-Ju; Hepworth, Shelley R.; Wang, Suo-Min] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Key Lab Grassland Livestock Ind Innovat, Minist Agr & Rural Affairs, Lanzhou 730020, Gansu, Peoples R China; [Hepworth, Shelley R.] Carleton Univ, Inst Biochem, Dept Biol, Ottawa, ON, Canada
Reprint Address Wang, SM (corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China.; Wang, SM (corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Key Lab Grassland Livestock Ind Innovat, Minist Agr & Rural Affairs, Lanzhou 730020, Gansu, Peoples R China.
E-mail Address smwang@lzu.edu.cn
ORCID Number Hepworth, Shelley/0000-0002-6496-3792
Funding Agency and Grant Number National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730093, 31470503]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [lzujbky-2018-k01]
Funding Text This research was supported by the National Natural Science Foundation of China (31730093 and 31470503) and the Fundamental Research Funds for the Central Universities (lzujbky-2018-k01).
Times Cited 4
Total Times Cited Count (WoS, BCI, and CSCD) 5
Publisher CSIRO PUBLISHING
Publisher City CLAYTON
Publisher Address UNIPARK, BLDG 1, LEVEL 1, 195 WELLINGTON RD, LOCKED BAG 10, CLAYTON, VIC 3168, AUSTRALIA
ISSN 1445-4408
29-Character Source Abbreviation FUNCT PLANT BIOL
ISO Source Abbreviation Funct. Plant Biol.
Year Published 2019
Volume 46
Issue 7
Beginning Page 670
Ending Page 683
Digital Object Identifier (DOI) 10.1071/FP18295
Page Count 14
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number IK7TS
Unique Article Identifier WOS:000476795600007
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