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Authors Baeshen, MN; Ahmed, F; Moussa, TAA; Abulfaraj, AA; Jalal, RS; Noor, SO; Baeshen, NA; Huelsenbeck, JP
Author Full Name Baeshen, M. N.; Ahmed, F.; Moussa, T. A. A.; Abulfaraj, A. A.; Jalal, R. S.; Noor, S. O.; Baeshen, N. A.; Huelsenbeck, J. P.
Title A COMPARATIVE ANALYSIS OF DE NOVO TRANSCRIPTOME ADAPTATION OF DESERT PLANTS IN SAUDI ARABIA
Source APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
Language English
Document Type Article
Author Keywords plant transcriptome; GO; KEGG; simple sequence repeats; heat stress; drought stress; salinity factors; transcription factors
Keywords Plus HEAT-SHOCK PROTEINS; RNA-SEQ DATA; GENE-EXPRESSION; RHAZYA-STRICTA; DROUGHT STRESS; SSR MARKERS; GENOME; ARABIDOPSIS; RESPONSES; IDENTIFICATION
Abstract Rhazya stricta, Senna italica, and Zygophyllum simplex are important desert plants of Saudi Arabia with great economic and medicinal value. However, their tolerance and survival mechanisms under combined abiotic stresses such as high temperature, high salinity, and drought are not well understood. In order to investigate the potential molecule mechanism of abiotic stress tolerance in these plants, we used de novo transcriptome assembly and their comparative analysis. This study used leaf tissues to construct three cDNA libraries of these plants and then generated RNA-seq data by the Illumina HiSeq2000 platform. Sequencing reads were de novo assembled to generate: (a) 71,116 unigenes in R. stricta; (b) 59,274 unigenes in S. italica; (c) 70,300 unigenes in Z. simplex. Furthermore, the unigenes from these plants were annotated and analyzed with different databases. A comparative analysis of KEGG pathways identified several common pathways induced in these plants, including & ldquo;Plant-pathogen interaction & rdquo;, & ldquo;Plant hormone signal transduction & rdquo;, & ldquo;Spliceosome & rdquo;, & ldquo;RNA transport & rdquo;, and & ldquo;Protein processing in endoplasmic reticulum & rdquo;, which may play an important role in combined abiotic drought, heat, and salinity stress. Finally, a comparative analysis of transcriptional regulators identified C2H2, C3H, CCHC(Zn), MYB-HB-like, PHD, WD40-like, and bHLH as common Transcription Factors responsible for abiotic stress tolerance in these plants. Our study revealed key factors involved in abiotic stress tolerance, which could be applied to develop high-yield transgenic crops capable of growing under combined abiotic stresses in the field.
Author Address [Baeshen, M. N.; Moussa, T. A. A.; Jalal, R. S.; Huelsenbeck, J. P.] Univ Jeddah, Coll Sci, Dept Biol, Jeddah, Saudi Arabia; [Ahmed, F.] Univ Jeddah, Coll Sci, Dept Biochem, Jeddah, Saudi Arabia; [Moussa, T. A. A.] Cairo Univ, Fac Sci, Bot & Microbiol Dept, Giza 12613, Egypt; [Abulfaraj, A. A.] King Abdulaziz Univ, Dept Biol Sci, Coll Sci & Arts, Rabigh Campus, Jeddah 21589, Saudi Arabia; [Noor, S. O.; Baeshen, N. A.] King Abdulaziz Univ, Fac Sci, Dept Biol, Jeddah, Saudi Arabia; [Huelsenbeck, J. P.] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA
Reprint Address Baeshen, MN (corresponding author), Univ Jeddah, Coll Sci, Dept Biol, Jeddah, Saudi Arabia.; Ahmed, F (corresponding author), Univ Jeddah, Coll Sci, Dept Biochem, Jeddah, Saudi Arabia.
E-mail Address mnbaeshen@uj.edu.sa; fahmed1@uj.edu.sa
ResearcherID Number Ahmed, Firoz/AAR-6971-2021; Baeshen, Mohammed Nabih/I-4398-2012
ORCID Number Ahmed, Firoz/0000-0003-4702-5144; Baeshen, Mohammed Nabih/0000-0002-0235-5222
Funding Agency and Grant Number Deanship of Scientific Research (DSR), University of Jeddah, Jeddah [UJ-01-18-ICP]; DSR
Funding Text This work was funded by the Deanship of Scientific Research (DSR), University of Jeddah, Jeddah, under grant No. (UJ-01-18-ICP). The authors, therefore, acknowledge with thanks DSR technical and financial support.
Publisher CORVINUS UNIV BUDAPEST
Publisher City BUDAPEST
Publisher Address VILLANYI UT 29/43, BUDAPEST, H-1118, HUNGARY
ISSN 1589-1623
29-Character Source Abbreviation APPL ECOL ENV RES
ISO Source Abbreviation Appl. Ecol. Environ. Res.
Year Published 2021
Volume 19
Issue 3
Beginning Page 1753
Ending Page 1782
Digital Object Identifier (DOI) 10.15666/aeer/1903_17531782
Page Count 30
Web of Science Category Ecology; Environmental Sciences
Subject Category Environmental Sciences & Ecology
Document Delivery Number SL4GN
Unique Article Identifier WOS:000656877100013
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