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Authors Cifuentes, A; Anton, J; de Wit, R; Rodriguez-Valera, F
Author Full Name Cifuentes, A; Anton, J; de Wit, R; Rodriguez-Valera, F
Title Diversity of Bacteria and Archaea in sulphate-reducing enrichment cultures inoculated from serial dilution of Zostera noltii rhizosphere samples
Source ENVIRONMENTAL MICROBIOLOGY
Language English
Document Type Article
Keywords Plus 16S RIBOSOMAL-RNA; MARINE SURFACE SEDIMENT; NITROGEN-FIXATION RATES; SP-NOV; DESULFOBACTERIUM-PHENOLICUM; PHYLOGENETIC IDENTIFICATION; REDUCTIVE DECHLORINATION; PROKARYOTES; OXIDATION; ROOTS
Abstract We have analysed the diversity of culturable sulphate-reducing bacteria (SRB) in Zostera noltii colonized sediments from Bassin d'Arcachon (France). Four organic substrates have been tested as well as the combination of H(2) and CO(2) to select for lithotrophic SRB. All energy sources were supplied in parallel cultures that were amended with yeast extract plus NH(4)(+) and prepared without a source of combined nitrogen, the latter to select for diazotrophic SRB. The 10 different enrichment media were inoculated from serial dilution of rhizosphere samples. The highest dilution cultures yielding positive growth (i.e. 10(-7)) were studied by molecular techniques (16S rDNA clone libraries, RISA and ARDRA). Lactate as a single organic substrate in combination with a source of combined nitrogen resulted in selection of members of the Desulfovibrionaceae. Surprisingly, when lactate was added without a source of combined nitrogen, Desulfobacteriaceae were selected. A strong influence of the presence or absence of combined nitrogen was also observed for the substrates sucrose and fructose. Whereas the liquid culture growing on sucrose and NH(4)(+) systematically yielded 16S rDNA clones related to an environmental unidentified green sulphur bacterium (OPS185), on plates we were able to isolate a SRB related to Desulfovibrio dechloracetivorans, which likely represents a non-described species. Under diazotrophic conditions, sucrose selected for SRB clones related to the cluster formed by Desulfovibrio zosterae, Desulfovibrio salexigens and Desulfovibrio bastinii. The corresponding isolate obtained on plates showed only low sequence similarity with this closest neighbour (93.8%), and we suggest that it also represents a non-described species. Surprisingly, a 16S rDNA sequence corresponding to an archaeon, i.e. a non-extremophile Crenoarchaeota , was retrieved from several of the SRB enrichment cultures even after subsequent transfers.
Author Address Univ Alicante, Dept Fisiol Genet & Microbiol, Div Microbiol, E-03080 Alicante, Spain; Univ Miguel Hernandez, Dept Prod Vegetal & Microbiol, Div Microbiol, Alacant, Spain; Univ Bordeaux 1, CNRS, UMR 5805, Lab Oceanog Biol, Arcachon, France
Reprint Address Anton, J (corresponding author), Univ Alicante, Dept Fisiol Genet & Microbiol, Div Microbiol, Apto 99,San Vicente del Raspeig, E-03080 Alicante, Spain.
E-mail Address anton@ua.es
ResearcherID Number Rodriguez-Valera, Francisco/M-2782-2013; Anton, Josefa/L-5283-2014
ORCID Number Rodriguez-Valera, Francisco/0000-0002-9809-2059; Anton, Josefa/0000-0002-5823-493X
Times Cited 16
Total Times Cited Count (WoS, BCI, and CSCD) 19
Publisher WILEY-BLACKWELL PUBLISHING, INC
Publisher City MALDEN
Publisher Address COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
ISSN 1462-2912
29-Character Source Abbreviation ENVIRON MICROBIOL
ISO Source Abbreviation Environ. Microbiol.
Publication Date SEP
Year Published 2003
Volume 5
Issue 9
Beginning Page 754
Ending Page 764
Digital Object Identifier (DOI) 10.1046/j.1470-2920.2003.00470.x
Page Count 11
Web of Science Category Microbiology
Subject Category Microbiology
Document Delivery Number 711LZ
Unique Article Identifier WOS:000184744200005
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