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| Authors | Koce, JD; Vilhar, B; Bohanec, B; Dermastia, M |
| Author Full Name | Koce, JD; Vilhar, B; Bohanec, B; Dermastia, M |
| Title | Genome size of Adriatic seagrasses |
| Source | AQUATIC BOTANY |
| Language | English |
| Document Type | Article |
| Author Keywords | seagrasses; Alismatidae; genome size; C-value; DNA image cytometry |
| Keywords Plus | NUCLEAR-DNA CONTENT; CHROMOSOME-NUMBERS; MARINE ANGIOSPERMS; FLOW-CYTOMETRY; DENSITOMETRY; EVOLUTION; PLANTS |
| Abstract | Genome size (C-value) was measured in four species of Adriatic seagrasses with interphase-peak DNA image cytometry. The estimated 2C-value was 1.5 pg DNA for Zostera noltii (2n = 12), 1.2 pg for Zostera marina (2n = 12), 1.1 pg for Cymodocea nodosa (2n = 28) and 6.2 pg for Posidonia oceanica, using Pisuin sativum (2C-value = 8.84 pg) as the calibration standard. Seagrass leaves were fixed in 4% buffered formaldehyde to mitigate stoichiometric error due to tannins and post-fixed in 3:1 methanol:acetic acid (MAA). DNA was stained with the Feulgen reaction after hydrolysis in 5 M HCl for 90 min at 20 degreesC. Comparison of genome size of seagrasses with the data for other species of Alismatidae indicated that the ancestral genome of Alismatidae was relatively small. (C) 2003 Elsevier B.V. All rights reserved. |
| Author Address | Univ Ljubljana, Biotech Fac, Dept Biol, SI-1001 Ljubljana, Slovenia; Univ Ljubljana, Biotech Fac, Dept Agron, SI-1001 Ljubljana, Slovenia |
| Reprint Address | Koce, JD (corresponding author), Univ Ljubljana, Biotech Fac, Dept Biol, Vecna Pot 111, SI-1001 Ljubljana, Slovenia. |
| Times Cited | 23 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 24 |
| Publisher | ELSEVIER SCIENCE BV |
| Publisher City | AMSTERDAM |
| Publisher Address | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| ISSN | 0304-3770 |
| 29-Character Source Abbreviation | AQUAT BOT |
| ISO Source Abbreviation | Aquat. Bot. |
| Publication Date | SEP |
| Year Published | 2003 |
| Volume | 77 |
| Issue | 1 |
| Beginning Page | 17 |
| Ending Page | 25 |
| Digital Object Identifier (DOI) | 10.1016/S0304-3770(03)00072-X |
| Page Count | 9 |
| Web of Science Category | Plant Sciences; Marine & Freshwater Biology |
| Subject Category | Plant Sciences; Marine & Freshwater Biology |
| Document Delivery Number | 718NH |
| Unique Article Identifier | WOS:000185152700002 |
| Plants associated with this reference |