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Authors Koce, JD; Vilhar, B; Bohanec, B; Dermastia, M
Author Full Name Koce, JD; Vilhar, B; Bohanec, B; Dermastia, M
Title Genome size of Adriatic seagrasses
Source AQUATIC BOTANY
Language English
Document Type Article
Author Keywords seagrasses; Alismatidae; genome size; C-value; DNA image cytometry
Keywords Plus NUCLEAR-DNA CONTENT; CHROMOSOME-NUMBERS; MARINE ANGIOSPERMS; FLOW-CYTOMETRY; DENSITOMETRY; EVOLUTION; PLANTS
Abstract Genome size (C-value) was measured in four species of Adriatic seagrasses with interphase-peak DNA image cytometry. The estimated 2C-value was 1.5 pg DNA for Zostera noltii (2n = 12), 1.2 pg for Zostera marina (2n = 12), 1.1 pg for Cymodocea nodosa (2n = 28) and 6.2 pg for Posidonia oceanica, using Pisuin sativum (2C-value = 8.84 pg) as the calibration standard. Seagrass leaves were fixed in 4% buffered formaldehyde to mitigate stoichiometric error due to tannins and post-fixed in 3:1 methanol:acetic acid (MAA). DNA was stained with the Feulgen reaction after hydrolysis in 5 M HCl for 90 min at 20 degreesC. Comparison of genome size of seagrasses with the data for other species of Alismatidae indicated that the ancestral genome of Alismatidae was relatively small. (C) 2003 Elsevier B.V. All rights reserved.
Author Address Univ Ljubljana, Biotech Fac, Dept Biol, SI-1001 Ljubljana, Slovenia; Univ Ljubljana, Biotech Fac, Dept Agron, SI-1001 Ljubljana, Slovenia
Reprint Address Koce, JD (corresponding author), Univ Ljubljana, Biotech Fac, Dept Biol, Vecna Pot 111, SI-1001 Ljubljana, Slovenia.
Times Cited 23
Total Times Cited Count (WoS, BCI, and CSCD) 24
Publisher ELSEVIER SCIENCE BV
Publisher City AMSTERDAM
Publisher Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN 0304-3770
29-Character Source Abbreviation AQUAT BOT
ISO Source Abbreviation Aquat. Bot.
Publication Date SEP
Year Published 2003
Volume 77
Issue 1
Beginning Page 17
Ending Page 25
Digital Object Identifier (DOI) 10.1016/S0304-3770(03)00072-X
Page Count 9
Web of Science Category Plant Sciences; Marine & Freshwater Biology
Subject Category Plant Sciences; Marine & Freshwater Biology
Document Delivery Number 718NH
Unique Article Identifier WOS:000185152700002
Plants associated with this reference

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