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Authors Brodersen, KE; Siboni, N; Nielsen, DA; Pernice, M; Ralph, PJ; Seymour, J; Kuhl, M
Author Full Name Brodersen, Kasper Elgetti; Siboni, Nachshon; Nielsen, Daniel A.; Pernice, Mathieu; Ralph, Peter J.; Seymour, Justin; Kuhl, Michael
Title Seagrass rhizosphere microenvironment alters plant-associated microbial community composition
Source ENVIRONMENTAL MICROBIOLOGY
Language English
Document Type Article
Keywords Plus EELGRASS ZOSTERA-MARINA; RADIAL OXYGEN LOSS; SULFATE REDUCTION; TROPICAL SEAGRASS; SULFIDE INTRUSION; NITROGEN-FIXATION; ORGANIC-CARBON; O-2 DYNAMICS; SEDIMENT; ROOTS
Abstract The seagrass rhizosphere harbors dynamic microenvironments, where plant-driven gradients of O-2 and dissolved organic carbon form microhabitats that select for distinct microbial communities. To examine how seagrass-mediated alterations of rhizosphere geochemistry affect microbial communities at the microscale level, we applied 16S rRNA amplicon sequencing of artificial sediments surrounding the meristematic tissues of the seagrass Zostera muelleri together with microsensor measurements of the chemical conditions at the basal leaf meristem (BLM).Radial O-2 loss (ROL) from the BLM led to approximate to 300 mu m thick oxic microzones, wherein pronounced decreases in H2S and pH occurred. Significantly higher relative abundances of sulphate-reducing bacteria were observed around the meristematic tissues compared to the bulk sediment, especially around the root apical meristems (RAM; approximate to 57% of sequences). Within oxic microniches, elevated abundances of sulphide-oxidizing bacteria were observed compared to the bulk sediment and around the RAM. However, sulphide oxidisers within the oxic microzone did not enhance sediment detoxification, as rates of H2S re-oxidation here were similar to those observed in a pre-sterilized root/rhizome environment. Our results provide novel insights into how chemical and microbiological processes in the seagrass rhizosphere modulate plant-microbe interactions potentially affecting seagrass health.
Author Address [Brodersen, Kasper Elgetti; Siboni, Nachshon; Nielsen, Daniel A.; Pernice, Mathieu; Ralph, Peter J.; Seymour, Justin; Kuhl, Michael] UTS, Fac Sci, Climate Change Cluster, Sydney, NSW, Australia; [Brodersen, Kasper Elgetti; Kuhl, Michael] Univ Copenhagen, Dept Biol, Marine Biol Sect, Helsingor, Denmark
Reprint Address Brodersen, KE (corresponding author), UTS, Fac Sci, Climate Change Cluster, Sydney, NSW, Australia.; Brodersen, KE (corresponding author), Univ Copenhagen, Dept Biol, Marine Biol Sect, Helsingor, Denmark.
E-mail Address kasper.elgetti.brodersen@bio.ku.dk
ResearcherID Number Kuhl, Michael/A-1977-2009; Pernice, Mathieu/AAU-9285-2020; Ralph, Peter J/C-5029-2009; Siboni, Nachshon/K-4202-2012; Ralph, Peter/L-1527-2019; Nielsen, Daniel/AAU-1065-2020
ORCID Number Kuhl, Michael/0000-0002-1792-4790; Ralph, Peter J/0000-0002-3103-7346; Siboni, Nachshon/0000-0001-6082-0949; Ralph, Peter/0000-0002-3103-7346; Nielsen, Daniel/0000-0001-6678-5937; Pernice, Mathieu/0000-0002-3431-2104; Seymour, Justin/0000-0002-3745-6541; Brodersen, Kasper Elgetti/0000-0001-9010-1179
Funding Agency and Grant Number Australian Research CouncilAustralian Research Council [LP110200454, FT130100218]; Carlsberg FoundationCarlsberg Foundation [CF16-0899]; Augustinus Foundation; P.A. Fiskers Fund; Danish Council for Independent Research Natural SciencesDet Frie Forskningsrad (DFF)
Funding Text We thank Jessica Tout and Jean-Baptiste Raina from the University of Technology Sydney for fruitful discussions and support during experiments. The research project was funded by grants from the Australian Research Council (LP110200454 to MK, JS and PR and FT130100218 to JS), the Carlsberg Foundation (CF16-0899 to KEB), the Augustinus Foundation (KEB), P.A. Fiskers Fund (KEB) and a Sapere-Aude Advanced grant from the Danish Council for Independent Research Natural Sciences (MK).
Times Cited 28
Total Times Cited Count (WoS, BCI, and CSCD) 29
Publisher WILEY
Publisher City HOBOKEN
Publisher Address 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
ISSN 1462-2912
29-Character Source Abbreviation ENVIRON MICROBIOL
ISO Source Abbreviation Environ. Microbiol.
Publication Date AUG
Year Published 2018
Volume 20
Issue 8
Special Issue SI
Beginning Page 2854
Ending Page 2864
Digital Object Identifier (DOI) 10.1111/1462-2920.14245
Page Count 11
Web of Science Category Microbiology
Subject Category Microbiology
Document Delivery Number GU3NA
Unique Article Identifier WOS:000445184600013
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