Loading content, please wait..
loading..
Logo
Version 3.22
or
Publication Type J
Authors Dave, A., P. Sanadhya, P. S. Joshi, P. Agarwal and P. K. Agarwal
Title Molecular cloning and characterization of high-affinity potassium transporter (AlHKT2;1) gene promoter from halophyte Aeluropus lagopoides
Source International Journal of Biological Macromolecules
Author Keywords Aeluropus lagopoides Salt stress AlHKT2 1 Promoter-deletions MUG assay cis-elements salt tolerance transcription factors functional-analysis hkt transporters expression rice na+ drought mechanisms responses
Abstract HKT subfamily II functions as Na+ - K+ co-transporter and prevents plants from salinity stress. A 760 bp promoter region of AlHKT2:1 was isolated, sequenced and cloned. The full length promoter D1, has many cis-regulatory elements like MYB, MBS, W box, ABRE etc. involved in abiotic stress responses. D1 and subsequent 5' deletions were cloned into pCAMBIA1301 and studied for its efficacy in stress conditions in heterologous system. Blue colour staining was observed in flower petals, anther lobe, and dehiscence slit of anther in To plants. The T-1 seedlings showed staining in leaf veins, shoot vasculature and root except root tip. T-1 seedlings were subjected to NaCl, KCl, NaCl + KCl and ABA stresses. GUS activity was quantified by 4-methylumbelliferyl glucuronide (4-MUG) assay under control and stress conditions. The smallest deletion- D4 also showed GUS expression but highest activity was observed in D2 as compared to full length promoter and other deletions. The electrophoretic mobility shift assay using stress-induced protein with different promoter deletions revealed more prominent binding in D2. These results suggest that AlHKT2:1 promoter is involved in abiotic stress response and deletion D2 might be sufficient to drive the stress-inducible expression of various genes involved in providing stress tolerance in plants. (C) 2021 Elsevier B.V. All rights reserved.
Author Address [Dave, Ankita; Sanadhya, Payal; Joshi, Priyanka S.; Agarwal, Parinita; Agarwal, Pradeep K.] Council Sci & Ind Res CSIR, Div Plant Omics, CSIR Cent Salt & Marine Chem Res Inst CSIR CSMCRI, Gijubhai Badheka Marg, Bhavnagar 364002, Gujarat, India. [Dave, Ankita; Joshi, Priyanka S.; Agarwal, Pradeep K.] Acad Sci & Innovat Res AcSIR, Ghaziabad 201002, India. Agarwal, PK (corresponding author), Council Sci & Ind Res CSIR, Div Plant Omics, CSIR Cent Salt & Marine Chem Res Inst CSIR CSMCRI, Gijubhai Badheka Marg, Bhavnagar 364002, Gujarat, India. pagarwal@csmcri.res.in
ISSN 0141-8130
ISBN 0141-8130
29-Character Source Abbreviation Int. J. Biol. Macromol.
Publication Date Jun
Year Published 2021
Volume 181
Beginning Page 1254-1264
Digital Object Identifier (DOI) 10.1016/j.ijbiomac.2021.05.038
Unique Article Identifier WOS:000656888300005

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed