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Publication Type J
Authors Christakis, C. A., G. Daskalogiannis, A. Chatzaki, E. A. Markakis, G. Mermigka, A. Sagia, G. F. Rizzo, V. Catara, I. Lagkouvardos, D. J. Studholme and P. F. Sarris
Title Endophytic Bacterial Isolates From Halophytes Demonstrate Phytopathogen Biocontrol and Plant Growth Promotion Under High Salinity
Source Frontiers in Microbiology
Author Keywords halophytes endophytes stress tolerance salinity tolerance biofertilizers biocontrol bio-inoculants growth-promotion sp nov. stress tolerance root microbiota acc deaminase michiganensis salt virulence database
Abstract Halophytic endophytes potentially contribute to the host's adaptation to adverse environments, improving its tolerance against various biotic and abiotic stresses. Here, we identified the culturable endophytic bacteria of three crop wild relative (CWR) halophytes: Cakile maritima, Matthiola tricuspidata, and Crithmum maritimum. In the present study, the potential of these isolates to improve crop adaptations to various stresses was investigated, using both in vitro and in-planta approaches. Endophytic isolates were identified by their 16S rRNA gene sequence and evaluated for their ability to: grow in vitro in high levels of NaCl; inhibit the growth of the economically important phytopathogens Verticillium dahliae, Ralstonia solanacearum, and Clavibacter michiganensis and the human pathogen Aspergillus fumigatus; provide salt tolerance in-planta; and provide growth promoting effect in-planta. Genomes of selected isolates were sequenced. In total, 115 endophytic isolates were identified. At least 16 isolates demonstrated growth under increased salinity, plant growth promotion and phytopathogen antagonistic activity. Three showed in-planta suppression of Verticillium growth. Furthermore, representatives of three novel species were identified: two Pseudomonas species and one Arthrobacter. This study provides proof-of-concept that the endophytes from CWR halophytes can be used as "bio-inoculants," for the enhancement of growth and stress tolerance in crops, including the high-salinity stress.
Author Address [Christakis, Christos A.; Mermigka, Glykeria; Sarris, Panagiotis F.] Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, Iraklion, Greece. [Daskalogiannis, Georgia; Sagia, Angeliki; Sarris, Panagiotis F.] Univ Crete, Dept Biol, Iraklion, Greece. [Chatzaki, Anastasia; Markakis, Emmanouil A.] Hellen Agr Org DIMITRA, Inst Olive Tree Subtrop Crops & Viticulture, Dept Viticulture Vegetable Crops Floriculture & P, Lab Mycol, Iraklion, Greece. [Rizzo, Giulio Flavio; Catara, Vittoria] Univ Catania, Dept Agr Food & Environm, Catania, Italy. [Lagkouvardos, Ilias] Tech Univ Munich, ZIEL Inst Food & Hlth, Freising Weihenstephan, Germany. [Studholme, David J.; Sarris, Panagiotis F.] Univ Exeter, Biosci, Exeter, Devon, England. Sarris, PF (corresponding author), Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, Iraklion, Greece.; Sarris, PF (corresponding author), Univ Crete, Dept Biol, Iraklion, Greece.; Sarris, PF (corresponding author), Univ Exeter, Biosci, Exeter, Devon, England. p.sarris@imbb.forth.gr
ISSN 1664-302X
ISBN 1664-302X
29-Character Source Abbreviation Front. Microbiol.
Publication Date May
Year Published 2021
Volume 12
Digital Object Identifier (DOI) 10.3389/fmicb.2021.681567
Unique Article Identifier WOS:000651396900001

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