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Authors Mu, DY; Zwiazek, JJ; Li, ZQ; Zhang, WQ
Author Full Name Mu, Deyu; Zwiazek, Janusz J.; Li, Zhouqi; Zhang, Wenqing
Title Genotypic variation in salt tolerance of Ulmus pumila plants obtained by shoot micropropagation
Source ACTA PHYSIOLOGIAE PLANTARUM
Language English
Document Type Article
Author Keywords Genotypic variation; Salt-tolerance selection; Ulmus pumila; Micropropagation
Keywords Plus IN-VITRO SELECTION; SODIUM-CHLORIDE; PHOTOSYNTHETIC CHARACTERISTICS; MESOPHYLL CHLOROPLASTS; DROUGHT TOLERANCE; PICEA-MARIANA; SIBERIAN ELM; GROWTH; SALINITY; STRESS
Abstract Siberian elm (Ulmus pumila L.) is a tree native to Asia and is widely cultivated in the other parts of the world due to its resistance to drought, temperature extremes, and salinity. In this study, we obtained nine clones of Siberian elm by in vitro shoot propagation of trees varying in growth, morphological characteristics, and salt tolerance in the field to examine traits linked to salt tolerance. The plantlets were subjected for 2 weeks to 0, 0.3, 0.5, 0.7, 0.8, and 0.9 % (w/v) NaCl and to mixture of salts representative of sea water (NaCl, CaCl, K2SO4, MgSO4, and NaHCO3) to examine the effects on growth, tissue concentrations of major osmolytes, proteins, and chlorophyll, as well as activities of the antioxidant enzymes. While higher salt treatment (both NaCl and mixed salt from 0.7 to 0.9 %) concentrations decreased growth rates in all of the studied clones, 0.3 % NaCl treatment resulted in greater shoot growth parameters of the treated plantlets compared with untreated control. In mixed salt treatments, growth parameters, including shoot relative elongation rate (SRGR), branching rate (BR), and length of new shoots (SL), decreased in the examined clones with the exception of clones 51, 105, and 65225, respectively, in which treatment concentrations as high as 0.3 % NaCl had no effect on shoot length. In both NaCl and mixed salt treatments, the antioxidant enzymes (SOD, POD, and CAT) in most of these clones increased at 0.3-0.7 % (or 0.8 %) treatment concentrations and decreased at 0.8-0.9 % salt treatments; malondialdehyde (MDA) content increased with the growing of the salt concentrations; soluble protein and chlorophyll content show a decreased tendency with the raising of the salt concentrations; in most elm clones, sugar and free proline (Pro) content accumulate at 0.3-0.8 % salt treatment decreased in 0.9 % salt treatment. In mixed salt treatment, the superoxide (SOD) shows more activities in most of the clones compared with NaCl treatment. In most clones, chlorophyll concentrations were greater in NaCl compared with mixed salt treatment in 0.8-0.9 % concentrations; the decrease of soluble protein concentrations in the mixed salt was lower compared with NaCl treatment. Using the membership function and cluster analyses, the clones were classified into four distinctly different groups in terms of their salt tolerance. The differences in responses of the examined physiological parameters to salt treatments between these clones with different salt-tolerance capacities point to the importance of osmoregulation and maintenance of antioxidant enzyme activities in salt tolerance of Siberian elm.
Author Address [Mu, Deyu; Li, Zhouqi] Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China; [Zwiazek, Janusz J.; Zhang, Wenqing] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2E3, Canada
Reprint Address Li, ZQ (corresponding author), Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China.
E-mail Address lizhouqi@nwsuaf.edu.cn
ResearcherID Number Zwiazek, Janusz/AAP-2543-2021
ORCID Number Zhang, Wen-Qing/0000-0003-1604-7471
Funding Agency and Grant Number World Bank loan project (Shandong Ecological Afforestation Project) [P112759, SEAP-PY-3]; China Scholarship Council (CSC)China Scholarship Council
Funding Text We acknowledge funding support from the World Bank loan project (Shandong Ecological Afforestation Project P112759, Funding No.: SEAP-PY-3). We thank the China Scholarship Council (CSC) for providing funding to Deyu Mu in the form of living allowance to study in J. J. Zwiazek's laboratory. We also thank Dr. Chen Ding for help with the data analysis.
Times Cited 4
Total Times Cited Count (WoS, BCI, and CSCD) 4
Publisher SPRINGER HEIDELBERG
Publisher City HEIDELBERG
Publisher Address TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
ISSN 0137-5881
29-Character Source Abbreviation ACTA PHYSIOL PLANT
ISO Source Abbreviation Acta Physiol. Plant.
Publication Date AUG
Year Published 2016
Volume 38
Issue 8
Article Number 188
Digital Object Identifier (DOI) 10.1007/s11738-016-2189-4
Page Count 16
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number DS0EH
Unique Article Identifier WOS:000380267700002
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