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Authors Zhu, JF; Yang, XY; Liu, ZX; Zhang, HX
Author Full Name Zhu, Jianfeng; Yang, Xiuyan; Liu, Zhengxiang; Zhang, Huaxin
Title Identification and Target Prediction of MicroRNAs in Ulmus pumila L. Seedling Roots under Salt Stress by High-Throughput Sequencing
Source FORESTS
Language English
Document Type Article
Author Keywords Ulmus pumila L.; MicroRNA; MicroRNA target; salt stress; high-throughput sequencing; qRT-PCR
Keywords Plus GENOME-WIDE IDENTIFICATION; DIFFERENTIAL EXPRESSION; ARABIDOPSIS-THALIANA; DEVELOPMENTAL-STAGES; DEGRADOME ANALYSIS; MESSENGER-RNA; GENES; PLANT; TOLERANCE; GERMINATION
Abstract MicroRNAs (miRNAs) are a class of endogenous small RNAs with important roles in plant growth, development, and environmental stress responses. Ulmus pumila L., a deciduous broadleaved tree species of northern temperate regions, is widely distributed in central and northern Asia and has important economic and ecological value. With the spread and aggravation of soil salinization, salt stress has become a major abiotic stress affecting the normal growth and development of U. pumila. However, the influence of salt stress on U. pumila miRNA expression has not been investigated. To identify miRNAs and predict their target mRNA genes under salt stress, three small RNA libraries were generated and sequenced from roots of U. pumila seedlings treated with various concentrations of NaCl corresponding to no salt stress, light short-term salt stress, and medium-heavy long-term salt stress. Integrative analysis identified 254 conserved miRNAs representing 29 families and 49 novel miRNAs; 232 potential targets of the miRNAs were also predicted. Expression profiling of miRNAs between libraries was performed, and the expression of six miRNAs was validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Our findings provide an overview of potential miRNAs and corresponding targets involved in regulating U. pumila salt defense responses. These results lay the foundation for further research into molecular mechanisms involved in salt stress resistance in U. pumila and other Ulmaceae species.
Author Address [Zhu, Jianfeng; Yang, Xiuyan; Liu, Zhengxiang; Zhang, Huaxin] State Forestry Adm, Res Ctr Saline & Alkali Land, Beijing 100091, Peoples R China
Reprint Address Zhang, HX (corresponding author), State Forestry Adm, Res Ctr Saline & Alkali Land, Beijing 100091, Peoples R China.
E-mail Address jianfengzhu666@163.com; sueyxy@126.com; xiangzilz@163.com; zhanghx1998@126.com
ORCID Number Zhu, Jianfeng/0000-0001-8308-6505
Funding Agency and Grant Number Fundamental Research Funds for the Central Non-profit Research Institution of CAF [CAFYBB2014QA040]; National Key Research and Development Program of China [2016YFC0501303]
Funding Text The authors are grateful to the anonymous reviewers for their constructive comments. This research was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (Project No. CAFYBB2014QA040) and the National Key Research and Development Program of China (Project No. 2016YFC0501303).
Times Cited 3
Total Times Cited Count (WoS, BCI, and CSCD) 5
Publisher MDPI
Publisher City BASEL
Publisher Address ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
ISSN 1999-4907
29-Character Source Abbreviation FORESTS
ISO Source Abbreviation Forests
Publication Date DEC
Year Published 2016
Volume 7
Issue 12
Article Number 318
Digital Object Identifier (DOI) 10.3390/f7120318
Page Count 13
Web of Science Category Forestry
Subject Category Forestry
Document Delivery Number EJ6KI
Unique Article Identifier WOS:000393327700015
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