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Authors Jeong, HN; Sun, HJ; Zuo, ZF; Lee, DH; Song, PS; Kang, HG; Lee, HY
Author Full Name Jeong, Ha-Na; Sun, Hyeon-Jin; Zuo, Zhi-Fang; Lee, Dong Hee; Song, Pill-Soon; Kang, Hong-Gyu; Lee, Hyo-Yeon
Title Overexpression of ATHG1/AHL23 and ATPG3/AHL20, Arabidopsis AT-hook motif nuclear-localized genes, confers salt tolerance in transgenic Zoysia japonica
Source PLANT BIOTECHNOLOGY REPORTS
Language English
Document Type Article
Author Keywords Zoysia japonica; AT-hook motif nuclear-localized (AHL); ATHG1; AHL23; ATPG3; AHL20; Overexpression; Salt tolerance
Keywords Plus OXIDATIVE STRESS; HYPOCOTYL GROWTH; PROTEIN; SALINITY; QUALITY; WATER; PHOTOSYNTHESIS; EXPRESSION; INTERACTS; CHROMATIN
Abstract Zoysia japonica Steud. is a native turfgrass in the Korean peninsula and is used worldwide. Abnormal conditions of soil salinity inhibit plant growth and adversely affect the quality of the turfgrass. AT-hook motif nuclear-localized (AHL) family proteins known to co-regulate the transcription of genes as a chromatin remodeling factor play a role in plant developmental processes and stress responses. In this study, AT-Hook Protein of Genomine 1 (ATHG1)/AT-Hook motif nuclear-Localized protein 23 (AHL23) and AT-hook Protein of Genomine 3 (ATPG3)/AT-Hook motif nuclear-Localized protein 20 (AHL20), two AHL genes from A. thaliana, were transformed into Z. japonica under the control of a constitutive ubiquitin promoter. Southern blot analysis proved that ATHG1/AHL23 and ATPG3/AHL20 were introduced into the ATHG1/AHL23-transgenic plants and ATPG3/AHL20-transgenic plants, respectively. Overexpression of each ATHG1/AHL23 and ATPG3/AHL20 in all of the transgenic plants was confirmed by quantitative real-time PCR (qRT-PCR). To evaluate a tolerant response to salt stress of the transgenic plants, 4 transgenic plants including ATHG1-overexpressing line 1 (ATHG1-OE1) and ATHG1-OE2, and ATPG3-overexpressing line 1 (ATPG3-OE1) and ATPG3-OE2 were selected, respectively. All the transgenic plants showed higher salt-tolerant phenotype with higher chlorophyll and lower malondialdehyde (MDA) contents under salt treatment, compared to the wild types. Also, under salt treatment, the transgenic plants revealed higher activities of catalase (CAT) and peroxidase (POD), reactive oxygen species (ROS)-scavenging enzymes, than those of the wild-type plant. These results suggest that overexpression of ATHG1/AHL23 or ATPG3/AHL20 belonging to the AHL gene family confers salt tolerance to the transgenic zoysiagrass plants.
Author Address [Jeong, Ha-Na; Zuo, Zhi-Fang; Lee, Hyo-Yeon] Jeju Natl Univ, Fac Biotechnol, Jeju 690756, South Korea; [Sun, Hyeon-Jin; Song, Pill-Soon; Kang, Hong-Gyu; Lee, Hyo-Yeon] Jeju Natl Univ, Subtrop Hort Res Inst, Jeju 690756, South Korea; [Jeong, Ha-Na] Quegenbiotech Co Ltd, Incheon 429931, South Korea; [Lee, Dong Hee] Genomine Inc, Genomine Adv Biotechnol Res Inst, Pohang 37668, South Korea
Reprint Address Lee, HY (corresponding author), Jeju Natl Univ, Fac Biotechnol, Jeju 690756, South Korea.; Kang, HG; Lee, HY (corresponding author), Jeju Natl Univ, Subtrop Hort Res Inst, Jeju 690756, South Korea.
E-mail Address honggyu@jejunu.ac.kr; hyoyeon@jejunu.ac.kr
ORCID Number , Hong-Gyu/0000-0002-2428-2757
Funding Agency and Grant Number Bio-Green 21 Program of Rural Development AdministrationRural Development Administration (RDA) [PJ01368501]; National Research Foundation of Korea (NRF) of the Ministry of Education, Republic of Korea [2019R1A6A1A11052070]
Funding Text This research was supported in part by grants from Bio-Green 21 Program of Rural Development Administration (PJ01368501) and from Basic Science Research Program through the National Research Foundation of Korea (NRF) of the Ministry of Education (2019R1A6A1A11052070), Republic of Korea.
Publisher SPRINGER
Publisher City NEW YORK
Publisher Address ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES
ISSN 1863-5466
29-Character Source Abbreviation PLANT BIOTECHNOL REP
ISO Source Abbreviation Plant Biotechnol. Rep.
Publication Date JUN
Year Published 2020
Volume 14
Issue 3
Beginning Page 351
Ending Page 361
Digital Object Identifier (DOI) 10.1007/s11816-020-00606-9
Page Count 11
Web of Science Category Biotechnology & Applied Microbiology; Plant Sciences
Subject Category Biotechnology & Applied Microbiology; Plant Sciences
Document Delivery Number LR2TJ
Unique Article Identifier WOS:000515945600001
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