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Authors Bejaoui, F; Salas, JJ; Nouairi, I; Smaoui, A; Abdelly, C; Martinez-Force, E; Ben Youssef, N
Author Full Name Bejaoui, Fatma; Salas, Joaquin J.; Nouairi, Issam; Smaoui, Abderrazak; Abdelly, Chedly; Martinez-Force, Enrique; Ben Youssef, Nabil
Title Changes in chloroplast lipid contents and chloroplast ultrastructure in Sulla carnosa and Sulla coronaria leaves under salt stress
Source JOURNAL OF PLANT PHYSIOLOGY
Language English
Document Type Article
Author Keywords Chloroplast lipids; Fatty acids; Lipid peroxidation; Sulla carnosa; Sulla coronaria; Ultrastructure
Keywords Plus MEMBRANE-LIPIDS; WATER-STRESS; OXIDATIVE-STRESS; BRASSICA-NAPUS; NACL SALINITY; TOLERANCE; CADMIUM; PLANTS; ACCUMULATION; TOXICITY
Abstract The possible involvement of chloroplast lipids in the mechanisms of NaCl tolerance was studied in leaves of two varieties of Fabaceae: Sulla carnosa and Sulla coronaria, which were subjected to 200 mM NaCl over 20 days. Changes in membrane lipid peroxidation, chloroplast lipids content, fatty acids (FA) composition and the ultrastructure of chloroplasts under salt stress were investigated. Chloroplast lipids were separated and quantified by high performance liquid chromatography coupled to evaporative light scattering detection (HPLC/ELSD). The results showed that salinity induced a significant decrease in digalactosyldiacylglycerol (DGDG), phosphatidylglycerol (PG) and sulfoquinovosylglycerol (SQDG) content in both S. carnosa and S. coronaria leaves, whereas monogalactosyldiacylglycerol (MGDG) content did not change significantly in S. carnosa leaves. The MGDG/DGDG ratio remained stable in S. coronaria leaves but increased in those of S. carnosa. In addition, the unsaturated-to-saturated fatty acids ratio (UFAs:SFAs) did not change under salt stress in S. coronaria leaves, while it decreased significantly in S. carnosa leaves. Moreover, salinity did not induce significant changes in MGDG and DGDG unsaturation level in S. carnosa leaves, in contrast to S. coronaria, in which salinity seems to enhance the unsaturation level in MGDG, DGDG and PG. Furthermore, the level of membrane lipid peroxidation, as expressed by malondialdehyde (MDA) levels, increased at 200mM in S. carnosa leaves, while it did not change significantly in those of S. coronaria. With respect to the ultrastructure of chloroplasts at 200mM NaCl, investigated by transmission electron microscopy (TEM), salt-stress caused the swelling of thylakoids in S. carnosa mesophyll. These ultrastructural changes were observed especially in the spongy tissue in S. coronaria. Taken together, these findings suggest that the stability of MGDG/DGDG ratio, the unchanged unsaturation level, and increasing unsaturation level in MGDG, DGDG and PG may be effective to some degree in suppressing the ultrastructural damage caused by salinity effects and may contribute to protect the chloroplast membrane integrity against salt stress. (C) 2016 Elsevier GmbH. All rights reserved.
Author Address [Bejaoui, Fatma; Smaoui, Abderrazak; Abdelly, Chedly] CBBC, LEP, POB 901, Hammam Lif 2050, Tunisia; [Salas, Joaquin J.; Martinez-Force, Enrique] CSIC, Inst Grasa, Edificio 46,Campus Univ Pablo de Olavide, Seville 41013, Spain; [Ben Youssef, Nabil] CBBC, Lab Olive Biotechnol, POB 901, Hammam Lif 2050, Tunisia; [Nouairi, Issam] CBBC, Lab Legumes, POB 901, Hammam Lif 2050, Tunisia
Reprint Address Bejaoui, F (corresponding author), CBBC, LEP, POB 901, Hammam Lif 2050, Tunisia.
E-mail Address bjaouifatma@gmail.com
ResearcherID Number Issam, Nouairi/M-3506-2019; Martinez-Force, Enrique/A-7979-2008; Nouairi, Issam/E-1820-2013; Linan, Joaquin Jesus Salas/AAT-5738-2020; , Nabil/ABB-7663-2020
ORCID Number Issam, Nouairi/0000-0003-1850-1164; Martinez-Force, Enrique/0000-0001-5324-9537; Nouairi, Issam/0000-0003-1850-1164; Linan, Joaquin Jesus Salas/0000-0002-3259-2587; , Nabil/0000-0003-2597-8067
Funding Agency and Grant Number Ministry of Higher Education and Scientific Research of Tunisia
Funding Text This work was funded by the Ministry of Higher Education and Scientific Research of Tunisia. Imaging was performed at the Bordeaux Imaging Center, member of the national infrastructure France Biolmaging.
Times Cited 25
Total Times Cited Count (WoS, BCI, and CSCD) 27
Publisher ELSEVIER GMBH
Publisher City MUNICH
Publisher Address HACKERBRUCKE 6, 80335 MUNICH, GERMANY
ISSN 0176-1617
29-Character Source Abbreviation J PLANT PHYSIOL
ISO Source Abbreviation J. Plant Physiol.
Publication Date JUL 1
Year Published 2016
Volume 198
Beginning Page 32
Ending Page 38
Digital Object Identifier (DOI) 10.1016/j.jplph.2016.03.018
Page Count 7
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number DN1IX
Unique Article Identifier WOS:000376820600004
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