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Authors Hidri, R; Metoui-Ben Mahmoud, O; Debez, A; Abdelly, C; Barea, JM; Azcon, R
Author Full Name Hidri, Rabaa; Metoui-Ben Mahmoud, Ouissal; Debez, Ahmed; Abdelly, Chedly; Barea, Jose-Miguel; Azcon, Rosario
Title Modulation of C:N:P stoichiometry is involved in the effectiveness of a PGPR and AM fungus in increasing salt stress tolerance of Sulla carnosa Tunisian provenances
Source APPLIED SOIL ECOLOGY
Language English
Document Type Article
Author Keywords Bacillus subtilis; Rhizophagus intraradices; Sulla carnosa provenances; Nutrition; C:N:P stoichiometry; Salinity
Keywords Plus GROWTH-PROMOTING RHIZOBACTERIA; ARBUSCULAR MYCORRHIZAL FUNGI; PLANT-GROWTH; BACTERIAL COMMUNITY; SOIL; TOMATO; INOCULATION; COLONIZATION; RHIZOSPHERE; MECHANISMS
Abstract Sulla carnosa (Hedysarum carnosum Desf.) is an important legume grown as animal feed on salt-affected areas. Here, we assess the influence of plant-growth-promoting bacteria (PGPR) (Bacillus subtilis) with or without the mycorrhizal fungus (AMF) Rhizophagus intraradices on four S. carnosa Tunisian provenances (Sidi khlif, Thelja, Kalbia and Kerker) grown in the greenhouse under saline and non-saline conditions. Plant growth, mineral nutrition, C:N:P stoichiometry in plant shoots, and soil enzymatic activities were considered. Microbial effectiveness on plant (roots and shoots) growth ranged from insignificant in Kerker to highly positive in the provenances Sidi khlif and Kalbia. In the latter provenance, the positive effect on growth was more pronounced under dual than single inoculation. Plant growth declined significantly under salt stress compared to the control, but Sidi khlif provenance was the most severely impacted. Especially in Kalbia, shoot mineral (N, P, K, Mg, Cu, and Fe) concentrations increased significantly in salt-stressed plants inoculated with R. intraradices as compared to the salt-stressed and non-inoculated plants. This suggests that inoculation with R. intraradices during nursery establishment contributes to salt stress alleviation by maintaining a favourable nutrient profile in Kalbia provenance. Shoots of Sidi khlif plants inoculated with B. subtilis demonstrated distinct C:N:P stoichiometry as compared to salt-treated and non-inoculated plants, indicating that B. subtilis could increase plant tolerance to salt stress through nutrient allocation patterns. The efficiency of B. subtilis in Kalbia and Thelja was due to the high IAA production by this strain under stress conditions. Application of PGPR and AMF improved soil quality by increasing the activities of soil enzyme such as urease, alkaline phosphatase, beta-glucosidase and dehydrogenase compared to their respective non-inoculated controls. From this study, we conclude that microbial inoculation was necessary for S. carnosa provenances to reach their optimal nutrition under saline and non-saline conditions. Thus, the use of adequate microbial inocula and provenance could be a critical issue for the successful ecological stability of this Mediterranean legume, thereby improving its suitability for the remediation of saline lands.
Author Address [Hidri, Rabaa; Metoui-Ben Mahmoud, Ouissal; Debez, Ahmed; Abdelly, Chedly] Biotechnol Ctr Borj Cedria, Lab Extremophile Plants, BP 901, Hammam Lif 2050, Tunisia; [Hidri, Rabaa; Barea, Jose-Miguel; Azcon, Rosario] CSIC, Dept Microbiol Suelo & Sistemas Simbiot, Estn Expt Zaidin, Prof Albareda 1, E-18008 Granada, Spain
Reprint Address Hidri, R (corresponding author), Biotechnol Ctr Borj Cedria, Lab Extremophile Plants, BP 901, Hammam Lif 2050, Tunisia.
E-mail Address hidrirabaa@gmail.com
ResearcherID Number Debez, Ahmed/F-3714-2014
ORCID Number Debez, Ahmed/0000-0002-6786-7544
Funding Agency and Grant Number Ministry of Economy and Competitiveness (Spain) [AGL2009-12530-C02-02]; Tunisian Ministry of Higher Education and Scientific Research [LR10CBBC02]
Funding Text This work was carried out in the framework of project AGL2009-12530-C02-02 from Ministry of Economy and Competitiveness (Spain) and supported by the Tunisian Ministry of Higher Education and Scientific Research (LR10CBBC02), for a stay at the Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion Experimental del Zaidin, CSIC, Granada, Spain. We are grateful to Prof. Bacem Mnasri for critically reading the manuscript.
Times Cited 6
Total Times Cited Count (WoS, BCI, and CSCD) 6
Publisher ELSEVIER
Publisher City AMSTERDAM
Publisher Address RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS
ISSN 0929-1393
29-Character Source Abbreviation APPL SOIL ECOL
ISO Source Abbreviation Appl. Soil Ecol.
Publication Date NOV
Year Published 2019
Volume 143
Beginning Page 161
Ending Page 172
Digital Object Identifier (DOI) 10.1016/j.apsoil.2019.06.014
Page Count 12
Web of Science Category Soil Science
Subject Category Agriculture
Document Delivery Number IT0TY
Unique Article Identifier WOS:000482561100019
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