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Authors Jothiramshekar, S; Benjamin, JJ; Krishnasamy, R; George, S; Swaminathan, R; Parida, A
Author Full Name Jothiramshekar, Saranya; Benjamin, Jenifer Joseph; Krishnasamy, Rani; George, Suja; Swaminathan, Rajalakshmi; Parida, Ajay
Title Identification of salt-responsive genes from C4 halophyte Suaeda nudiflora through suppression subtractive hybridization and expression analysis under individual and combined treatment of salt and elevated carbon dioxide conditions
Source PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
Language English
Document Type Article
Author Keywords Suaeda nudiflora; C4 photosynthesis; Suppression subtractive hybridization; Semi quantitative PCR; Salt and elevated carbon dioxide conditions
Keywords Plus SEQUENCE TAGS; LIPID-PEROXIDATION; SALINITY STRESS; CO2 CONCENTRATION; AVICENNIA-MARINA; WATER STATUS; GROWTH; L.; LEAVES; PHOTOSYNTHESIS
Abstract Salinization of soil is a prime abiotic stress that limits agriculture productivity worldwide. To Study the mechanisms that halophytes take up to survive under high salt condition is important in engineering salinity stress tolerance in sensitive species. Suaeda nudiflora is a halophyte plant that grows in the saline environment and extreme high tidal belt. The species have high capability to produce high protein biomass in salty soils due to C4 photosynthesis. The physiological and biochemical changes in S. nudiflora under salinity stress were studied by measuring chlorophyll content, electrolytic leakage, level of lipid peroxidation and total soluble sugars. Increased lipid peroxidation and electrolytic leakage was observed in salt stressed S. nudiflora compared to control plants. A suppression subtractive hybridization strategy was employed to identify differentially expressed genes under salt treatment in S. nudiflora. A total of 333 positive clones were identified and screened. Of these, 250 expressed sequence tags were identified. cDNA subtraction library resulted in 33 contigs and 138 singletons. The functional annotation and metabolic pathways identification were performed using the Blast2GO program. In addition, we analyzed the expression patterns of 18 genes associated with salt stress-responsive pathways by semi-quantitative PCR under salt and elevated carbon dioxide (CO2) conditions. Several of the analyzed genes showed an increase in expression levels under different time points of salt treatment and at different concentrations of salt. When the same genes were studied for its expression under elevated CO2 concentrations, most of the known salt responsive genes showed higher expression under the combined treatment of elevated CO2 concentrations (500 ppm) and NaCl treatment (200 mM) compare to ambient condition. This implies that salt responsive genes are enhanced at elevated CO2 concentrations.
Author Address [Jothiramshekar, Saranya; Benjamin, Jenifer Joseph; Krishnasamy, Rani; George, Suja; Swaminathan, Rajalakshmi] MS Swaminathan Res Fdn, Chennai, Tamil Nadu, India; [Parida, Ajay] Inst Life Sci, Bhubaneswar, India
Reprint Address George, S (corresponding author), MS Swaminathan Res Fdn, Chennai, Tamil Nadu, India.
E-mail Address sujageorge@mssrf.res.in
ORCID Number George, Suja/0000-0003-1066-273X
Publisher SPRINGER
Publisher City NEW YORK
Publisher Address ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES
ISSN 0971-5894
29-Character Source Abbreviation PHYSIOL MOL BIOL PLA
ISO Source Abbreviation Physiol. Mol. Biol. Plants
Publication Date JAN
Year Published 2020
Volume 26
Issue 1
Beginning Page 163
Ending Page 172
Digital Object Identifier (DOI) 10.1007/s12298-019-00722-z
Page Count 10
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number KN9GE
Unique Article Identifier WOS:000499229400002
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