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Authors Gong, Y; Bai, JL; Yang, HT; Zhang, WD; Xiong, YW; Ding, P; Qin, S
Author Full Name Gong, Yuan; Bai, Juan-Luan; Yang, Huan-Ting; Zhang, Wen-Di; Xiong, You-Wei; Ding, Peng; Qin, Sheng
Title Phylogenetic diversity and investigation of plant growth-promoting traits of actinobacteria in coastal salt marsh plant rhizospheres from Jiangsu, China
Source SYSTEMATIC AND APPLIED MICROBIOLOGY
Language English
Document Type Article
Author Keywords Coastal salt marsh; Actinobacteria; 16S rRNA gene; Diversity; Plant growth promoting
Keywords Plus SP NOV.; ACC DEAMINASE; STREPTOMYCES SPP.; BACTERIA; RHIZOBACTERIA; HALOPHYTE; SOILS; GENE; L.; XISHUANGBANNA
Abstract Actinobacteria from special habitats are of interest due to their producing of bioactive compounds and diverse ecological functions. However, little is known of the diversity and functional traits of actinobacteria inhabiting coastal salt marsh soils. We assessed actinobacterial diversity from eight coastal salt marsh rhizosphere soils from Jiangsu Province, China, using culture-based and 16S rRNA gene high throughput sequencing (HTS) methods, in addition to evaluating their plant growth-promoting (PGP) traits of isolates. Actinobacterial sequences represented 2.8%-43.0% of rhizosphere bacterial communities, as determined by HTS technique. The actinobacteria community comprised 34 families and 79 genera. In addition, 196 actinobacterial isolates were obtained, of which 92 representative isolates were selected for further 16S rRNA gene sequencing and phylogenetic analysis. The 92 strains comprised seven suborders, 12 families, and 20 genera that included several potential novel species. All representative strains were tested for their ability of producing indole acetic acid (IAA), siderophores, 1-aminocyclopropane- 1 -carboxylate deaminase (ACCD), hydrolytic enzymes, and phosphate solubilization. Based on the presence of multiple PGP traits, two strains, Streptomyces sp. KLBMP 50051 and Micromonospora sp. KLBMP 50019 were selected for inoculation of wheat seeds grown under salt stress. Both strains promoted seed germination, and KLBMP 50019 significantly enhanced seedling growth under NaC1 stress. Our study demonstrates that coastal salt marsh rhizosphere soils harbor a diverse reservoir of actinobacteria that are potential resources for the discovery of novel species and functions. Moreover, several of the isolates identified here are good candidates as PGP bacteria that may contribute to plant adaptions to saline soils. (C) 2018 Elsevier GmbH. All rights reserved.
Author Address [Gong, Yuan; Bai, Juan-Luan; Yang, Huan-Ting; Zhang, Wen-Di; Xiong, You-Wei; Ding, Peng; Qin, Sheng] Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plant Jiangsu Prov, Xuzhou 221116, Jiangsu, Peoples R China
Reprint Address Qin, S (corresponding author), Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plant Jiangsu Prov, Xuzhou 221116, Jiangsu, Peoples R China.
E-mail Address shengqin@jsnu.edu.cn
Funding Agency and Grant Number National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370062, 31000005]; Natural Science Foundation of the Jiangsu Higher Education Institutions of ChinaNational Natural Science Foundation of China (NSFC) [17KJA180004]; Qing Lan Project of Jiangsu Province; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Funding Text This work was supported by the National Natural Science Foundation of China (no. 31370062, 31000005), Natural Science Foundation of the Jiangsu Higher Education Institutions of China (17KJA180004), Qing Lan Project of Jiangsu Province (2014), and the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Times Cited 15
Total Times Cited Count (WoS, BCI, and CSCD) 16
Publisher ELSEVIER GMBH
Publisher City MUNICH
Publisher Address HACKERBRUCKE 6, 80335 MUNICH, GERMANY
ISSN 0723-2020
29-Character Source Abbreviation SYST APPL MICROBIOL
ISO Source Abbreviation Syst. Appl. Microbiol.
Publication Date SEP
Year Published 2018
Volume 41
Issue 5
Beginning Page 516
Ending Page 527
Digital Object Identifier (DOI) 10.1016/j.syapm.2018.06.003
Page Count 12
Web of Science Category Biotechnology & Applied Microbiology; Microbiology
Subject Category Biotechnology & Applied Microbiology; Microbiology
Document Delivery Number GT4JW
Unique Article Identifier WOS:000444471100011
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