Loading content, please wait..
loading..
Logo
Version 3.24
or
Authors Wang, FW; Wang, ML; Guo, C; Wang, N; Li, XW; Chen, H; Dong, YY; Chen, XF; Wang, ZM; Li, HY
Author Full Name Wang, F. W.; Wang, M. L.; Guo, C.; Wang, N.; Li, X. W.; Chen, H.; Dong, Y. Y.; Chen, X. F.; Wang, Z. M.; Li, H. Y.
Title Cloning and characterization of a novel betaine aldehyde dehydrogenase gene from Suaeda corniculata
Source GENETICS AND MOLECULAR RESEARCH
Language English
Document Type Article
Author Keywords BADH; Salt stress; Glycine betaine; Proline; SOD activity
Keywords Plus MOLECULAR-CLONING; STRESS TOLERANCE; GLYCINE BETAINE; CHOLINE MONOOXYGENASE; QUATERNARY AMMONIUM; DROUGHT RESISTANCE; ESCHERICHIA-COLI; ABIOTIC STRESS; SALT TOLERANCE; PLANTS
Abstract Glycine betaine is an important quaternary ammonium compound that is produced in response to several abiotic stresses in many organisms. The synthesis of glycine betaine requires the catalysis of betaine aldehyde dehydrogenase (BADH), which can convert betaine aldehyde into glycine betaine in plants, especially in halotolerant plants. In this study, we isolated the full-length cDNA of BADH from Suaeda corniculata (ScBADH) using reverse transcriptase-polymerase chain reaction and rapid amplification of cDNA ends. Next, we analyzed the expression profile of ScBADH using real-time PCR. The results showed that ScBADH expression was induced in the roots, stems, and leaves of S. corniculata seedlings under salt and drought stress. Next, ScBADH was overexpressed in Arabidopsis, resulting in the transgenic plants exhibiting enhanced tolerance over wild-type plants under salt and drought stress. We then analyzed the levels of glycine betaine and proline, as well as superoxide dismutase (SOD) activity, during salt stress in WT and transgenic Arabidopsis. The results indicated that overexpression of ScBADH produced more glycine betaine and proline, and increased SOD activity under NaCl treatment. Our results suggest that ScBADH might be a positive regulator in plants during the response to NaCl.
Author Address [Wang, F. W.; Wang, M. L.; Guo, C.; Wang, N.; Li, X. W.; Chen, H.; Dong, Y. Y.; Li, H. Y.] Jilin Agr Univ, Coll Life Sci, Engn Res Ctr, Chinese Minist Educ Bioreactor & Pharmaceut Dev, Changchun, Jilin, Peoples R China; [Chen, X. F.; Wang, Z. M.] Jilin Agr Univ, Jilin Technol Innovat Ctr Soybean Reg, Changchun, Jilin, Peoples R China
Reprint Address Li, HY (corresponding author), Jilin Agr Univ, Coll Life Sci, Engn Res Ctr, Chinese Minist Educ Bioreactor & Pharmaceut Dev, Changchun, Jilin, Peoples R China.
E-mail Address hyli99@163.com
Funding Agency and Grant Number Special Program for Research of Transgenic Plants [2014ZX08010-002]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31271746, 31201144, 31401403]
Funding Text Research supported by the Special Program for Research of Transgenic Plants (#2014ZX08010-002), and the National Natural Science Foundation of China (#31271746, #31201144 and #31401403).
Times Cited 8
Total Times Cited Count (WoS, BCI, and CSCD) 8
Publisher FUNPEC-EDITORA
Publisher City RIBEIRAO PRETO
Publisher Address RUA FLORIANO PEIXOTO 2444, ALTO DA BOA VISTA, RIBEIRAO PRETO, SP 00000, BRAZIL
ISSN 1676-5680
29-Character Source Abbreviation GENET MOL RES
ISO Source Abbreviation Genet. Mol. Res.
Year Published 2016
Volume 15
Issue 2
Article Number 15027848
Digital Object Identifier (DOI) 10.4238/gmr.15027848
Page Count 14
Web of Science Category Biochemistry & Molecular Biology; Genetics & Heredity
Subject Category Biochemistry & Molecular Biology; Genetics & Heredity
Document Delivery Number DY1UN
Unique Article Identifier WOS:000384880000068
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed