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Publication Type J
Authors Mann, A., N. Kumar, A. Kumar, C. Lata, A. Kumar, B. L. Meena, D. Mishra, M. Grover, S. Gaba, C. Parameswaran and N. Mantri
Title de novo transcriptomic profiling of differentially expressed genes in grass halophyte Urochondra setulosa under high salinity
Source Scientific Reports
Abstract Soil salinity is one of the major limiting factors for crop productivity across the world. Halophytes have recently been a source of attraction for exploring the survival and tolerance mechanisms at extreme saline conditions. Urochondra setulosa is one of the obligate grass halophyte that can survive in up to 1000 mM NaCl. The de novo transcriptome of Urochondra leaves at different salt concentrations of 300-500 mM NaCl was generated on Illumina HiSeq. Approximately 352.78 million high quality reads with an average contig length of 1259 bp were assembled de novo. A total of 120,231 unigenes were identified. On an average, 65% unigenes were functionally annotated to known proteins. Approximately 35% unigenes were specific to Urochondra. Differential expression revealed significant enrichment (P<0.05) of transcription factors, transporters and metabolites suggesting the transcriptional regulation of ion homeostasis and signalling at high salt concentrations in this grass. Also, about 143 unigenes were biologically related to salt stress responsive genes. Randomly selected genes of important pathways were validated for functional characterization. This study provides useful information to understand the gene regulation at extremely saline levels. The study offers the first comprehensive evaluation of Urochondra setulosa leaf transcriptome. Examining non-model organisms that can survive in harsh environment can provide novel insights into the stress coping mechanisms which can be useful to develop improved agricultural crops.
Author Address [Mann, Anita; Kumar, Naresh; Kumar, Ashwani; Lata, Charu; Kumar, Arvind; Meena, Babu Lal] ICAR Cent Soil Salin Res Inst, Karnal, Haryana, India. [Mishra, Dwijesh; Grover, Monendra; Gaba, Sonam] ICAR Indian Agr Stat Res Inst, New Delhi, India. [Parameswaran, C.] ICAR Natl Rice Res Inst, Cuttack, Odisha, India. [Mantri, Nitin] RMIT Univ, Sch Sci, Melbourne, Vic, Australia. Mann, A (corresponding author), ICAR Cent Soil Salin Res Inst, Karnal, Haryana, India. anitadgr13@gmail.com
ISSN 2045-2322
ISBN 2045-2322
29-Character Source Abbreviation Sci Rep
Publication Date Mar
Year Published 2021
Volume 11
Issue 1
Digital Object Identifier (DOI) 10.1038/s41598-021-85220-7
Unique Article Identifier WOS:000630440000016
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