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Authors Baisakh, N; Subudhi, PK; Arumuganathan, K; Parco, AP; Harrison, SA; Knott, CA; Materne, MD
Author Full Name Baisakh, Niranjan; Subudhi, Prasanta K.; Arumuganathan, K.; Parco, Arnold P.; Harrison, Stephen A.; Knott, Carrie A.; Materne, Michael D.
Title Development and interspecific transferability of genic microsatellite markers in Spartina spp. with different genome size
Source AQUATIC BOTANY
Language English
Document Type Article
Author Keywords DNA content; Expressed sequence tag; Polymorphism; Simple sequence repeat; Spartina; Transferability
Keywords Plus SIMPLE SEQUENCE REPEATS; NUCLEAR-DNA CONTENT; NATIVE S-FOLIOSA; EST-SSR MARKERS; GENETIC DIVERSITY; SALT STRESS; ALTERNIFLORA; WHEAT; RICE; LOCI
Abstract Flow cytometry analysis showed variation of nuclear DNA content among different species of Spartina. Spartina alterniflora had the biggest genome (1763.9 Mbp) and S. cynosuroides had the smallest genome (756.35 Mbp), whereas the genomes of S. patens (969.36 Mbp) and S. spartinae (979.78 Mbp) were comparable. Mining simple sequence repeats (SSR) from 1227 expressed sequence tags (EST) generated from salt stressed S. alterniflora showed an abundance of di- and tri-nucleotide repeats. Of 100 ESSR (EST-derived SSR) loci with five or more repeats, 81 loci were successfully amplified in eight S. alterniflora genotypes and 15 (22.2%) ESSR markers were polymorphic. Eleven of the 15 polymorphic ESSRs showed amplification across six different species of Spartina while 100% cross transferability was observed with at least one species of Spartina. The average number of alleles per marker was 3.9 and 5.8 within S. alterniflora and among Spartina species, respectively. The ESSR markers discriminated different members within and between species of Spartina genus. (C) 2009 Elsevier B.V. All rights reserved.
Author Address [Baisakh, Niranjan; Subudhi, Prasanta K.; Parco, Arnold P.; Harrison, Stephen A.; Knott, Carrie A.; Materne, Michael D.] Louisiana State Univ, Ctr Agr, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA; [Arumuganathan, K.] Virginia Mason Res Ctr, Flow & Image Cytometry Core Res Facil, Seattle, WA 98101 USA
Reprint Address Baisakh, N (corresponding author), Louisiana State Univ, Ctr Agr, Sch Plant Environm & Soil Sci, 104 MB Sturgis Hall, Baton Rouge, LA 70803 USA.
E-mail Address nbaisakh@agcenter.lsu.edu
Funding Agency and Grant Number USDA-CSRESS
Funding Text The authors acknowledge the help of Dr. Richard H. Neil and Garret P. Thomassie, USDA-NRCS, 6 Golden Meadow Plant Materials Center, Galliano, LA in collection of different species of Spartina. We thank Suman Andru for his help in POPGENE 1.32. This study was supported by a special grant from USDA-CSRESS. This manuscript is approved for publication by the Director of Louisiana Agricultural Experiment Station as manuscript number 2009-306-3643.
Times Cited 13
Total Times Cited Count (WoS, BCI, and CSCD) 13
Publisher ELSEVIER
Publisher City AMSTERDAM
Publisher Address RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS
ISSN 0304-3770
29-Character Source Abbreviation AQUAT BOT
ISO Source Abbreviation Aquat. Bot.
Publication Date NOV
Year Published 2009
Volume 91
Issue 4
Beginning Page 262
Ending Page 266
Digital Object Identifier (DOI) 10.1016/j.aquabot.2009.07.007
Page Count 5
Web of Science Category Plant Sciences; Marine & Freshwater Biology
Subject Category Plant Sciences; Marine & Freshwater Biology
Document Delivery Number 513RP
Unique Article Identifier WOS:000271341200002
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