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Authors Kocsis, MG; Nolte, KD; Rhodes, D; Shen, TL; Gage, DA; Hanson, AD
Author Full Name Kocsis, MG; Nolte, KD; Rhodes, D; Shen, TL; Gage, DA; Hanson, AD
Title Dimethylsulfoniopropionate biosynthesis in Spartina alterniflora - Evidence that S-methylmethionine and dimethylsulfoniopropylamine are intermediates
Source PLANT PHYSIOLOGY
Language English
Document Type Article
Keywords Plus DIAMINE OXIDASE; 3-DIMETHYLSULFONIOPROPIONATE; SUBCORDIFORMIS; PURIFICATION; ANGIOSPERMS; SALINITY; BIFLORA; SHOOTS; SULFUR; ORIGIN
Abstract The osmoprotectant 3-dimethylsulfoniopropionate (DMSP) occurs in Gramineae and Compositae, but its synthesis has been studied only in the latter. The DMSP synthesis pathway was therefore investigated in the salt marsh grass Spartina alterniflora Loisel. Leaf tissue metabolized supplied [S-35]methionine (Met) to S-methyl-L-Met (SMM), 3-dimethylsulfoniopropylamine (DMSP-amine), and DMSP. The S-35-labeling kinetics of SMM and DMSP-amine indicated that they were intermediates and, consistent with this, the dimethylsulfonium moiety of SMM was shown by stable isotope labeling to be incorporated as a unit into DMSP. The identity of DMSP-amine, a novel natural product, was confirmed by both chemical and mass-spectral methods. S. alterniflora readily converted supplied [S-35]SMM to DMSP-amine and DMSP, and also readily converted supplied [S-35]DMSP-amine to DMSP; grasses that lack DMSP did neither. A small amount of label was detected in 3-dimethylsulfoniopropionaldehyde (DMSP-ald) when [S-35]SMM or [S-35]DMSP-amine was given. These results are consistent with the operation of the pathway Met --> SMM --> DMSP-amine --> DMSP-ald --> DMSP, which differs from that found in Compositae by the presence of a free DMSP-amine intermediate. This dissimilarity suggests that DMSP synthesis evolved independently in Gramineae and Compositae.
Author Address Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA; Purdue Univ, Dept Hort, W Lafayette, IN 47907 USA; Michigan State Univ, Dept Biochem, E Lansing, MI 48824 USA
Reprint Address Hanson, AD (corresponding author), Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA.
Funding Agency and Grant Number NCRR NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Center for Research Resources (NCRR) [RR 00484] Funding Source: Medline
Times Cited 58
Total Times Cited Count (WoS, BCI, and CSCD) 69
Publisher AMER SOC PLANT PHYSIOLOGISTS
Publisher City ROCKVILLE
Publisher Address 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA
ISSN 0032-0889
29-Character Source Abbreviation PLANT PHYSIOL
ISO Source Abbreviation Plant Physiol.
Publication Date MAY
Year Published 1998
Volume 117
Issue 1
Beginning Page 273
Ending Page 281
Digital Object Identifier (DOI) 10.1104/pp.117.1.273
Page Count 9
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number ZN584
Unique Article Identifier WOS:000073660500030
Plants associated with this reference

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