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Authors Niu, DW; Li, SG; Yi, SZ; Shen, LH; Huang, MY; Zhou, RC; Tan, LC; Breitzig, M; Wang, F
Author Full Name Niu, Dewei; Li, Shuaiguang; Yi, Shanze; Shen, Lianghua; Huang, Meiyan; Zhou, Renchao; Tan, Lee Charles; Breitzig, Mason; Wang, Feng
Title Gene Cloning, Protein Expression and Functional Analysis of a type 3 Metallothionein with Bioaccumulation Potential from Sonneratia alba
Source POLISH JOURNAL OF ENVIRONMENTAL STUDIES
Language English
Document Type Article
Author Keywords bioaccumulation; Cu2+; metallothionein; metal tolerance; Pb2+; Sonneratia alba
Keywords Plus HEAVY-METALS; CADMIUM; PHYTOCHELATINS; EXPOSURE; PHYTOREMEDIATION; CONSUMPTION; BIOSORPTION; INDUCTION; TOLERANCE; SEDIMENT
Abstract Sonneratia alba (S. alba) is a mangrove species grown in brackish water of tropical and subtropical regions. Due to its unique environment, it has evolved various mechanisms for modulating salt and metal levels. In order to find the genes connected with bioaccumulation of metals, the root transcriptome annotation of Sonneratia alba was analyzed and a new metallothionein (MT) gene was cloned. Sequence analysis found that the new MT gene belongs to type 3 MT, which is mostly expressed in roots. A simple and efficient method was used to express the type 3 MT of S. alba (SaMT3) by transforming the recombinant expression vector pET15b-SaMT3 into Escherichia coli (E. coli) Rosetta-gami and induction with the optimal conditions of 500 mu M Isopropyl beta-D-1-thiogalactopyranoside (IPTG) at 24 degrees C for 12 h. OD600 of E. coli cells expressing His fused SaMT3 protein after treated with 500 mu M Cu2+ or 500 mu M Pb2+ for 12 h can reach 1.01 or 0.98, while OD600 of control cells expressing His-tag can reach only 0.81 or 0.75. Both control cells and the cells expressing SaMT3 accumulated metals. Cells expressing SaMT3, however, accumulated more Pb2+ and Cu2+ (more than two times) than control cells. In vivo, real-time PCR showed that the SaMT3 transcript was induced significantly when stimulated with 250 mu M, 500 mu M, or 1,000 mu M Cu2+ or Pb2+ for 24 h and 48 h. Taken together, the expression of SaMT3 can increase Cu2+ and Pb2+ resistance and binding capacity of E. coli.
Author Address [Niu, Dewei; Li, Shuaiguang; Yi, Shanze; Shen, Lianghua; Huang, Meiyan; Wang, Feng] Jinan Univ, Inst Genom Med, Coll Pharm, Guangzhou 510632, Guangdong, Peoples R China; [Niu, Dewei; Li, Shuaiguang; Yi, Shanze; Shen, Lianghua; Huang, Meiyan; Wang, Feng] Jinan Univ, Guangdong Prov Key Lab Pharmacodynam Constituents, Guangzhou 510632, Guangdong, Peoples R China; [Zhou, Renchao] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510530, Guangdong, Peoples R China; [Zhou, Renchao] Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resources, Guangzhou 510530, Guangdong, Peoples R China; [Tan, Lee Charles; Breitzig, Mason] Univ S Florida, Dept Internal Med, Morsani Coll Med, 12901 Bruce B Downs Blvd,MDC 19, Tampa, FL 33612 USA
Reprint Address Wang, F (corresponding author), Jinan Univ, Inst Genom Med, Coll Pharm, Guangzhou 510632, Guangdong, Peoples R China.; Wang, F (corresponding author), Jinan Univ, Guangdong Prov Key Lab Pharmacodynam Constituents, Guangzhou 510632, Guangdong, Peoples R China.
E-mail Address jnubiopharm@126.com
Funding Agency and Grant Number Traditional Chinese Medicine Bureau of Guangdong Province [20141065]; Science and Technology Innovation Project from Department of Education of Guangdong Province [2012KJCX0017]; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2017A030313561]
Funding Text This work was supported by the Traditional Chinese Medicine Bureau of Guangdong Province (NO. 20141065), Science and Technology Innovation Project from Department of Education of Guangdong Province (NO. 2012KJCX0017) and the Natural Science Foundation of Guangdong Province (NO. 2017A030313561).
Times Cited 2
Total Times Cited Count (WoS, BCI, and CSCD) 3
Publisher HARD
Publisher City OLSZTYN 5
Publisher Address POST-OFFICE BOX, 10-718 OLSZTYN 5, POLAND
ISSN 1230-1485
29-Character Source Abbreviation POL J ENVIRON STUD
ISO Source Abbreviation Pol. J. Environ. Stud.
Year Published 2018
Volume 27
Issue 5
Beginning Page 2203
Ending Page 2212
Digital Object Identifier (DOI) 10.15244/pjoes/75825
Page Count 10
Web of Science Category Environmental Sciences
Subject Category Environmental Sciences & Ecology
Document Delivery Number GI0KT
Unique Article Identifier WOS:000434059500030
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