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Authors Hasegawa, A; Oyanagi, T; Minagawa, R; Fujii, Y; Sasamoto, H
Author Full Name Hasegawa, Ai; Oyanagi, Tomoya; Minagawa, Reiko; Fujii, Yoshiharu; Sasamoto, Hamako
Title An inverse relationship between allelopathic activity and salt tolerance in suspension cultures of three mangrove species, Sonneratia alba, S. caseolaris and S. ovata: development of a bioassay method for allelopathy, the protoplast co-culture method
Source JOURNAL OF PLANT RESEARCH
Language English
Document Type Article
Author Keywords Allelopathy; Bioassay; Mangrove forest; Protoplast culture; Salt tolerance; Suspension culture
Keywords Plus GROWTH; CELLS
Abstract A bioassay method for allelopathy, the 'protoplast co-culture method' was developed to study the relationship between salt tolerance and allelopathy of three mangrove species, Sonneratia alba, S. caseolaris, and S. ovata. Plants of S. alba grow in the seaward-side high salinity region and plants of the latter two species grow in upstream-side regions of a mangrove forest, respectively. Effects of five sea salts (NaCl, KCl, MgCl2, MgSO4 and CaCl2) on the growth of the suspension cells of the latter two species were first investigated by a small-scale method using 24-well culture plates. S. ovata cells showed higher tolerance than S. caseolaris cells to NaCl and other salts, but were not as halophilic as S. alba cells. Protoplasts isolated from suspension cells were co-cultured with lettuce protoplasts in Murashige and Skoog's (MS) basal medium containing 1 mu M 2,4-dichlorophenoxyacetic acid, 0.1 mu M benzyladenine, 3 % sucrose and 0.6-0.8 M os-moticum. S. caseolaris protoplasts had a higher inhibitory effect on lettuce protoplast cell divisions than S. alba protoplasts at any lettuce protoplast density, and the effect of S. ovata was intermediate between the two. These results were similar to those obtained from a different in vitro bioassay method for allelopathy, the 'sandwich method' with dried leaves. The inverse relationship between allelopathic activity and salt tolerance in suspension cells of Sonneratia mangroves is discussed.
Author Address [Hasegawa, Ai; Oyanagi, Tomoya] Yokohama Natl Univ, Grad Sch Environm & Informat Sci, Yokohama, Kanagawa 2408501, Japan; [Minagawa, Reiko] Tokyo Univ Agr, Tokyo 1568502, Japan; [Fujii, Yoshiharu] Tokyo Univ Agr & Technol, Tokyo 1838509, Japan; [Sasamoto, Hamako] Yokohama Natl Univ, Fac Environm & Informat Sci, Yokohama, Kanagawa 2408501, Japan
Reprint Address Sasamoto, H (corresponding author), Yokohama Natl Univ, Fac Environm & Informat Sci, Yokohama, Kanagawa 2408501, Japan.
E-mail Address sasamoto@ynu.ac.jp
ResearcherID Number FUJII, Yoshiharu/C-8800-2013
ORCID Number FUJII, Yoshiharu/0000-0002-5412-395X
Times Cited 5
Total Times Cited Count (WoS, BCI, and CSCD) 5
Publisher SPRINGER JAPAN KK
Publisher City TOKYO
Publisher Address CHIYODA FIRST BLDG EAST, 3-8-1 NISHI-KANDA, CHIYODA-KU, TOKYO, 101-0065, JAPAN
ISSN 0918-9440
29-Character Source Abbreviation J PLANT RES
ISO Source Abbreviation J. Plant Res.
Publication Date NOV
Year Published 2014
Volume 127
Issue 6
Beginning Page 755
Ending Page 761
Digital Object Identifier (DOI) 10.1007/s10265-014-0651-1
Page Count 7
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number AS8EM
Unique Article Identifier WOS:000344482900009
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