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Authors NIKOLOPOULOS, D; MANETAS, Y
Author Full Name NIKOLOPOULOS, D; MANETAS, Y
Title COMPATIBLE SOLUTES AND INVITRO STABILITY OF SALSOLA-SODA ENZYMES - PROLINE INCOMPATIBILITY
Source PHYTOCHEMISTRY
Language English
Document Type Article
Author Keywords SALSOLA-SODA; CHENOPODIACEAE; COMPATIBLE SOLUTES; MALATE DEHYDROGENASE; NITRATE REDUCTASE; PHOSPHOENOLPYRUVATE CARBOXYLASE
Keywords Plus PHOSPHOENOLPYRUVATE CARBOXYLASE; ORGANIC COSOLUTES; SUAEDA-MARITIMA; PLANTS; GLYCINEBETAINE; INACTIVATION; POLYOLS; PROTEIN; STRESS
Abstract Proline is not compatible with enzymes extracted from Salsola soda, a glycine-betaine former adapted to saline soils. Proline accelerated thermal inactivation of nitrate reductase (EC 1.6.6.1), afforded no protection against dilution-induced inactivation of phosphoenolpyruvate carboxylase (EC 4.1.1.31) and only slightly opposed heat denaturation of NADH-dependent malate dehydrogenase (EC 1.1.1.37). Glycinebetaine, sorbitol and glycerol were, however, fully compatible, counteracting completely the adverse effects of heat and dilution. A co-evolution of enzyme structure and osmolyte systems, at least at the species level, is inferred from our results.
Reprint Address NIKOLOPOULOS, D (corresponding author), UNIV PATRAS,DEPT BIOL,PLANT PHYSIOL LAB,PATRAI,GREECE.
Times Cited 53
Total Times Cited Count (WoS, BCI, and CSCD) 58
Publisher PERGAMON-ELSEVIER SCIENCE LTD
Publisher City OXFORD
Publisher Address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB
ISSN 0031-9422
29-Character Source Abbreviation PHYTOCHEMISTRY
ISO Source Abbreviation Phytochemistry
Year Published 1991
Volume 30
Issue 2
Beginning Page 411
Ending Page 413
Digital Object Identifier (DOI) 10.1016/0031-9422(91)83694-G
Page Count 3
Web of Science Category Biochemistry & Molecular Biology; Plant Sciences
Subject Category Biochemistry & Molecular Biology; Plant Sciences
Document Delivery Number EW892
Unique Article Identifier WOS:A1991EW89200007
Plants associated with this reference

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