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Authors Borsodi, AK; Aszalos, JM; Bihari, P; Nagy, I; Schumann, P; Sproer, C; Kovacs, AL; Boka, K; Dobosy, P; Ovari, M; Szili-Kovacs, T; Toth, E
Author Full Name Borsodi, Andrea K.; Aszalos, Julia M.; Bihari, Peter; Nagy, Istvan; Schumann, Peter; Sproeer, Cathrin; Kovacs, Attila L.; Boka, Keroly; Dobosy, Peter; Ovari, Mihaly; Szili-Kovacs, Tibor; Toth, Erika
Title Anaerobacillus alkaliphilus sp. nov., a novel alkaliphilic and moderately halophilic bacterium
Source INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Language English
Document Type Article
Author Keywords Anaerobacillus; alkaliphilic and moderately halophilic bacterium; soda soil
Keywords Plus BACILLUS-ALKALIDIAZOTROPHICUS; FATTY-ACID; SODA LAKE; GEN. NOV.; DIVERSITY; SPECIATION; SEQUENCES; MACYAE; WATER
Abstract Two alkaliphilic and moderately halophilic bacterial strains B16-10(T) and Z23-18 characterized by optimal growth at pH 9.010.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunsag National Park, Hungary. Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia. The isolates were facultative anaerobic, catalase positive, oxidase negative. Both strains contained meso-diaminopimelic acid as diagnostic diaminoacid of the peptidoglycan. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone. Anteiso-C-15:0 , C(16:)(1)(w)11c and iso-C-14:0 were the major cellular fatty acids. The DNA G+C content of both strains was 35.8 mol%. The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10(T) and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006(T) (98.7 and 99.1 %), A. macyae JMM-4(T) (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6(T) (97.7 and 98.4%), A. alkalilacustris Z-0521(T) (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340(T) (97.5 and 98.2 %). However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10(T) and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp. nov. is proposed. The type strain is B16-10(T) (=DSM 29790(T)=NCAIM B 02608(T)).
Author Address [Borsodi, Andrea K.; Aszalos, Julia M.; Toth, Erika] Eotvos Lorand Univ, Dept Microbiol, Pazmany P Setany 1-C, H-1117 Budapest, Hungary; [Bihari, Peter; Nagy, Istvan] Seq Biotechnol Ltd, Vallalkozok Utja 7, H-6782 Morahalom, Hungary; [Nagy, Istvan] Hungarian Acad Sci, Biol Res Ctr, Inst Biochem, Temesvari Krt 62, H-6726 Szeged, Hungary; [Schumann, Peter; Sproeer, Cathrin] Leibniz Inst DSMZ German Collect Microorganisms &, Inhoffenstr 7 B, D-38124 Braunschweig, Germany; [Kovacs, Attila L.] Eotvos Lorand Univ, Dept Anat Cell & Dev Biol, Pazmany P Setany 1-C, H-1117 Budapest, Hungary; [Boka, Keroly] Eotvos Lorand Univ, Dept Plant Anat, Pazmany P Setany 1-C, H-1117 Budapest, Hungary; [Dobosy, Peter; Ovari, Mihaly] MTA Ctr Ecol Res, Danube Res Inst, Karolina Ut 29, H-1113 Budapest, Hungary; [Szili-Kovacs, Tibor] Hungarian Acad Sci, Agr Res Ctr, Inst Soil Sci & Agr Chem, Herman Otto Ut 15, H-1022 Budapest, Hungary
Reprint Address Borsodi, AK (corresponding author), Eotvos Lorand Univ, Dept Microbiol, Pazmany P Setany 1-C, H-1117 Budapest, Hungary.
E-mail Address borsodi.andrea@ttk.elte.hu
ResearcherID Number Borsodi, Andrea K./H-1148-2017; Szili-Kovacs, Tibor/AAJ-9680-2020; Schumann, Peter/AAL-1098-2020; Boka, Karoly/AAF-3466-2020
ORCID Number Borsodi, Andrea K./0000-0002-3738-7937; Szili-Kovacs, Tibor/0000-0002-8354-0656; Boka, Karoly/0000-0002-1324-3592
Funding Agency and Grant Number Hungarian Scientific Research FundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [NKFI-ID: K108572, UNKP-18-3]; New National Excellence Program of the Ministry of Human Capacities (Hungary)
Funding Text This research was supported by the Hungarian Scientific Research Fund (NKFI-ID: K108572). JM Aszalos was supported by the UNKP-18-3 New National Excellence Program of the Ministry of Human Capacities (Hungary).
Times Cited 2
Total Times Cited Count (WoS, BCI, and CSCD) 2
Publisher MICROBIOLOGY SOC
Publisher City LONDON
Publisher Address CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND
ISSN 1466-5026
29-Character Source Abbreviation INT J SYST EVOL MICR
ISO Source Abbreviation Int. J. Syst. Evol. Microbiol.
Publication Date MAR
Year Published 2019
Volume 69
Issue 3
Beginning Page 631
Ending Page 637
Digital Object Identifier (DOI) 10.1099/ijsem.0.003128
Page Count 7
Web of Science Category Microbiology
Subject Category Microbiology
Document Delivery Number HN4MF
Unique Article Identifier WOS:000460157300005
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