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Authors Gutierrez, AM; Cabriales, JJP; Vega, MM
Author Full Name Mauricio Gutierrez, Amparo; Pena Cabriales, Juan Jose; Maldonado Vega, Maria
Title Isolation and Characterization of Hexavalent Chromium-Reducing Rhizospheric Bacteria From a Wetland
Source INTERNATIONAL JOURNAL OF PHYTOREMEDIATION
Language English
Document Type Article
Author Keywords Bioremediation; bacterial consortia; Scirpus americanus Pers
Keywords Plus HYPERACCUMULATOR THLASPI-CAERULESCENS; OCHROBACTRUM-INTERMEDIUM; PLANT-GROWTH; CHROMATE REDUCTION; PSEUDOMONAS-PUTIDA; WASTE-WATER; CR(VI); REMOVAL; METAL; SULFATE
Abstract Scirpus americanus Pers. occurs naturally in oSan German,o a pond that serves as a receptor of industrial wastewater in Guanajuato, Mexico. This plant accumulates metals mainly in the root: concentrations (mg/kg) of Cr, As, Cd and Se were 970, 49, 41, and 85 respectively. Analysis of rhizosphere samples indicated bacterial population of 108 cfu g-1 in media with 0.2 mM Cr(VI) and 10 mM sodium gluconate. Thirteen isolates were obtained and phylogenetic analyses (16S rRNA) indicated they corresponded to genera of Agrobacterium, Arthrobacter, Microbacterium, Curtobacterium, Rhodococcus, Xanthomonas and Pseudomonas. Cr(VI) reduction was evaluated using the diphenyl carbazide method. The isolates accomplished 5-40% (20 M) of reduction in assays of resting cell and tolerated 0.5-5.0 mM Cr(VI). Eight strains used nitrate and thirteen used iron and chromium as electron acceptors to grow under anaerobic conditions. Cr(VI) reduction by five strains occurred at pH values (7-9) and NaCl concentrations (0.5-1.0 M) in basal medium. A mixed culture of strains (S17 and S28) reached a chromium removal of 100% at 0.2 mM Cr(VI) initial concentration. Aerobically, this consortium was capable of 93.8% Cr(VI) reduction of 81 gL-1 Cr(VI) of the industrial effluent, indicating their possible use in environmental cleanup.
Author Address [Mauricio Gutierrez, Amparo; Pena Cabriales, Juan Jose] Inst Politecn Nacl Campus Guanajuato, Ctr Invest & Estudios Avanzados, Irapuato 36500, Guanajuato, Mexico; [Maldonado Vega, Maria] Ctr Innovac Aplicada Tecnol Competit AC, Direcc Invest Ambiental, Leon, Guanajuato, Mexico
Reprint Address Cabriales, JJP (corresponding author), Inst Politecn Nacl Campus Guanajuato, Ctr Invest & Estudios Avanzados, Km 9-6 Libramiento Norte,Carretera Irapuato Leon, Irapuato 36500, Guanajuato, Mexico.
E-mail Address jpena@ira.cinvestav.mx
Funding Agency and Grant Number National Council of Science and Technology (Conacyt)Consejo Nacional de Ciencia y Tecnologia (CONACyT)
Funding Text This work was supported by National Council of Science and Technology (Conacyt). We thank Dr. Gene Bartholomew for valuable help in reviewing the manuscript. We extend our thanks to the anonymous reviewers for their positive and relevant comments that improved the manuscript.
Times Cited 16
Total Times Cited Count (WoS, BCI, and CSCD) 18
Publisher TAYLOR & FRANCIS INC
Publisher City PHILADELPHIA
Publisher Address 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA
ISSN 1522-6514
29-Character Source Abbreviation INT J PHYTOREMEDIAT
ISO Source Abbreviation Int. J. Phytoremediat.
Year Published 2010
Volume 12
Issue 4
Beginning Page 317
Ending Page 334
Article Number PII 918984830
Digital Object Identifier (DOI) 10.1080/15226510902968118
Page Count 18
Web of Science Category Environmental Sciences
Subject Category Environmental Sciences & Ecology
Document Delivery Number 593MU
Unique Article Identifier WOS:000277460600001
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