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Authors Ross, C; Puglisi, MP; Paul, VJ
Author Full Name Ross, Cliff; Puglisi, Melany P.; Paul, Valefie J.
Title Antifungal defenses of seagrasses from the Indian River Lagoon, Florida
Source AQUATIC BOTANY
Language English
Document Type Article
Author Keywords antifungal chemical defense; caspase; Indian River Lagoon; oxidative burst; programmed cell death; seagrass
Keywords Plus OXIDATIVE BURST; WASTING DISEASE; CELL-DEATH; ANTIMICROBIAL ACTIVITIES; THALASSIA-TESTUDINUM; SIGNAL-TRANSDUCTION; MARINE PATHOGENS; ZOSTERA-MARINA; RESISTANCE; PLANTS
Abstract We investigated the antifungal chemical defenses and physiological responses of five seagrasses collected from nearshore seagrass beds from the Indian River Lagoon, Florida, against a panel of co-occurring marine fungi isolated from nearby coastal communities. Whole plant tissues from Thalassia testudinum, Halodule wrightii and Syringodium filiforme prevented overgrowth by three of the seven fungi used in this study. Organic extracts from four of the five seagrasses inhibited the growth of at least one fungal strain. The extract from Ruppia maritima exhibited the highest antifungal activity, inhibiting the growth of three fungi including the pathogen Lindra thalassiae. Among the fungal panel, Fusarium sp. 2 was the most susceptible to seagrass extracts, whereas none of the extracts disrupted the growth of Dendryphiella salina and Fusarium sp. 3. Under laboratory conditions fungal inoculation elicited hydrogen peroxide production in all specimens within 25 min post-inoculation as measured with a redox sensitive dichlorodihydrofluorescein diacetate (DCFH-DA) assay. The concentration of H2O2 released into the immediate vicinity of infected seagrasses varied between 0.10 and 0.85 mu mol g(-1) FW min(-1) depending on seagrass species and pathogen combination. Longer term incubation (days) of T testudinum with homogenates of D. salina or L. thallasiae resulted in the induction of caspase activity, a known proteolytic activator of apoptotic and inflammatory activities. The application of micromolar concentrations of H2O2 to blades of T testudinum induced caspase activity suggesting that fungal detection, H2O2 production, and caspase activation occur in a consecutive order. The seagrasses examined in this study appear to use a combined strategy to combat fungal infection, including microbial chemical defenses and signaling pathways observed in terrestrial plants. Published by Elsevier B.V.
Author Address [Ross, Cliff; Puglisi, Melany P.; Paul, Valefie J.] Smithsonian Marine Stn, Ft Pierce, FL 34949 USA
Reprint Address Ross, C (corresponding author), Univ N Florida, Dept Biol, 1 UNF Dr, Jacksonville, FL 32224 USA.
E-mail Address cliff.ross@unf.edu
ResearcherID Number Ross, Cliff/B-8291-2011
Times Cited 25
Total Times Cited Count (WoS, BCI, and CSCD) 25
Publisher ELSEVIER SCIENCE BV
Publisher City AMSTERDAM
Publisher Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN 0304-3770
29-Character Source Abbreviation AQUAT BOT
ISO Source Abbreviation Aquat. Bot.
Publication Date FEB
Year Published 2008
Volume 88
Issue 2
Beginning Page 134
Ending Page 141
Digital Object Identifier (DOI) 10.1016/j.aquabot.2007.09.003
Page Count 8
Web of Science Category Plant Sciences; Marine & Freshwater Biology
Subject Category Plant Sciences; Marine & Freshwater Biology
Document Delivery Number 260PH
Unique Article Identifier WOS:000253021700007
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