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Authors Bharathkumar, S; RameshKumar, N; Paul, D; Prabavathy, VR; Nair, S
Author Full Name Bharathkumar, Srinivasan; RameshKumar, N.; Paul, Diby; Prabavathy, V. R.; Nair, Sudha
Title Characterization of the predominant bacterial population of different mangrove rhizosphere soils using 16S rRNA gene-based single-strand conformation polymorphism (SSCP)
Source WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Language English
Document Type Article
Author Keywords mangroves; PCR-SSCP; Bacillus; 16S r DNA gene
Keywords Plus PLANOCOCCUS-ALKANOCLASTICUS; LEAF-LITTER; SP-NOV.; COMMUNITIES; CARBON; DECOMPOSITION; RHIZOPHORA; DIVERSITY; SEDIMENT; DYNAMICS
Abstract Variations in chemical parameters and bacterial populations in mangrove rhizosphere samples were noted for different sites. The C, N, P and K contents as well as pH, EC and salinity showed variation between sites. Significant differences in soil properties were also found in sampling sites. Two types of soil were noted among sites. Guesthouse had significantly higher organic matter and nutrient content (N) than other three sites suggesting that human discharges, litter deposition and surface runoff were major nutrient inputs. This contaminated site was located at the landward edges. Positive correlations between organic matter, N, P and K contents were found suggesting that these nutrients were from similar input sources. Effects of sampling sites on microbial diversity were also analyzed via SSCP. Porteresia coarctata and Rhizophora mucronata did not show any variation in the banding patterns among replicates sampled in short distance within site. But Sonneratia apetala showed variation among replicates sampled in distance within site. A significant variation was noted in the SSCP profile among replicates between sites. The majority of dominant SSCP band sequences were related to bacterial genera of root and root-free soil environments, namely Bacillus, Planococcus, Planomicrobium, low G+C Gram-positive bacterium, glacial ice bacterium and unidentified bacteria. In the analysis of 16S rRNA sequences, members belonging to the phylum Firmicutes dominated the sequence collection. The phylogenetic analysis of 16S rRNA gene sequences showed close relationships to a wide range of clones or bacterial species of phylum Firmicutes and unidentified bacteria.
Author Address [Bharathkumar, Srinivasan; RameshKumar, N.; Paul, Diby; Prabavathy, V. R.; Nair, Sudha] MS Swaminathan Res Fdn, Madras 600113, Tamil Nadu, India
Reprint Address Nair, S (corresponding author), MS Swaminathan Res Fdn, Madras 600113, Tamil Nadu, India.
E-mail Address sudhanair@mssrf.res.in
ResearcherID Number PAUL, DIBY/G-8788-2014
ORCID Number PAUL, DIBY/0000-0002-9980-5516; Natatajan, Ramesh Kumar/0000-0003-1241-8933
Times Cited 13
Total Times Cited Count (WoS, BCI, and CSCD) 13
Publisher SPRINGER
Publisher City DORDRECHT
Publisher Address VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
ISSN 0959-3993
29-Character Source Abbreviation WORLD J MICROB BIOT
ISO Source Abbreviation World J. Microbiol. Biotechnol.
Publication Date MAR
Year Published 2008
Volume 24
Issue 3
Beginning Page 387
Ending Page 394
Digital Object Identifier (DOI) 10.1007/s11274-007-9487-3
Page Count 8
Web of Science Category Biotechnology & Applied Microbiology
Subject Category Biotechnology & Applied Microbiology
Document Delivery Number 265IN
Unique Article Identifier WOS:000253352400013
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