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Authors Menon, TG; Soniya, EV
Author Full Name Menon, Tara G.; Soniya, E. V.
Title Isolation and characterization of salt-induced genes from Rhizophora apiculata Blume, a true mangrove by suppression subtractive hybridization
Source CURRENT SCIENCE
Language English
Document Type Article
Author Keywords Gene expression; Rhizophora apiculata; salt stress; suppression subtractive hybridization
Keywords Plus BURMA MANGROVE; TOLERANCE; PLANT; STRESS
Abstract Mangroves are plants that can tolerate salinity equivalent to that present in seawater. In the present study, we have attempted to isolate the genes upregulated at the early stage of salt stress response in Rhizophora apiculata Blume, a true mangrove, through suppression subtractive hybridization technique. Several genes were isolated, which were then classified to nine functional categories, viz. metabolism, protein degradation and folding, secondary metabolism, cell rescue and defence, transport facilitation, signal transduction, transcription and translation, photosynthesis and unclassified genes of unknown function. The expression patterns of 12 of the genes thus obtained were studied at 6, 12 and 24 h time points of salinity stress using real-time PCR. Most genes were found to be upregulated under salt stress and showed maximum upregulation at the 6 h time point. Two of the genes studied were downregulated after 6 h, implying that the pattern of gene expression varies with time of application of stress. The genes thus isolated may be used to confer the trait of salt tolerance to non-tolerant genotypes, which can ultimately prove beneficial for crop improvement programmes.
Author Address [Menon, Tara G.; Soniya, E. V.] Rajiv Gandhi Ctr Biotechnol, Thiruvananthapuram 695014, Kerala, India
Reprint Address Soniya, EV (corresponding author), Rajiv Gandhi Ctr Biotechnol, Thycaud PO, Thiruvananthapuram 695014, Kerala, India.
E-mail Address evsoniya@rgcb.res.in
Funding Agency and Grant Number Department of Biotechnology, New DelhiDepartment of Biotechnology (DBT) India; Council of Scientific and Industrial Research, New Delhi, for awarding Senior Research Fellowship
Funding Text This work was supported by a grant from the Department of Biotechnology, New Delhi. T.G.M. thanks the Council of Scientific and Industrial Research, New Delhi, for awarding Senior Research Fellowship.
Times Cited 1
Total Times Cited Count (WoS, BCI, and CSCD) 1
Publisher INDIAN ACAD SCIENCES
Publisher City BANGALORE
Publisher Address C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA
ISSN 0011-3891
29-Character Source Abbreviation CURR SCI INDIA
ISO Source Abbreviation Curr. Sci.
Publication Date AUG 25
Year Published 2014
Volume 107
Issue 4
Beginning Page 650
Ending Page 655
Page Count 7
Web of Science Category Multidisciplinary Sciences
Subject Category Science & Technology - Other Topics
Document Delivery Number AU4ZL
Unique Article Identifier WOS:000345616700022
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