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Authors Kobayashi, S; Satone, H; Tan, EK; Kurokochi, H; Asakawa, S; Liu, SK; Takano, T
Author Full Name Kobayashi, Shio; Satone, Hina; Tan, Engkong; Kurokochi, Hiroyuki; Asakawa, Shuichi; Liu, Shenkui; Takano, Tetsuo
Title Transcriptional Responses of a Bicarbonate-Tolerant Monocot, Puccinellia tenuiflora, and a Related Bicarbonate-Sensitive Species, Poa annua, to NaHCO3 Stress
Source INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Language English
Document Type Article
Author Keywords Puccinellia tenuiflora; Poa annua; RNA-seq; alkaline salt stress; bicarbonate
Keywords Plus DIFFERENTIAL EXPRESSION ANALYSIS; GENE-EXPRESSION; IRON CHLOROSIS; ALKALI GRASS; ROOTS; RICE; K+; ACCUMULATION; NITRATE; GROWTH
Abstract Puccinellia tenuiflora is an alkaline salt-tolerant monocot found in saline-alkali soil in China. To identify the genes which are determining the higher tolerance of P. tenuiflora compared to bicarbonate sensitive species, we examined the responses of P. tenuiflora and a related bicarbonate-sensitive Poeae plant, Poa annua, to two days of 20 mM NaHCO3 stress by RNA-seq analysis. We obtained 28 and 38 million reads for P. tenuiflora and P. annua, respectively. For each species, the reads of both unstressed and stressed samples were combined for de novo assembly of contigs. We obtained 77,329 contigs for P. tenuiflora and 115,335 contigs for P. annua. NaHCO3 stress resulted in greater than two-fold absolute expression value changes in 157 of the P. tenuiflora contigs and 1090 of P. annua contigs. Homologs of the genes involved in Fe acquisition, which are important for the survival of plants under alkaline stress, were up-regulated in P. tenuiflora and down-regulated in P. annua. The smaller number of the genes differentially regulated in P. tenuiflora suggests that the genes regulating bicarbonate tolerance are constitutively expressed in P. tenuiflora.
Author Address [Kobayashi, Shio; Kurokochi, Hiroyuki; Takano, Tetsuo] Univ Tokyo, Asian Nat Environm Sci Ctr, Nishitokyo, Tokyo 1880002, Japan; [Satone, Hina; Tan, Engkong; Asakawa, Shuichi] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan; [Liu, Shenkui] Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Harbin 150040, Peoples R China
Reprint Address Takano, T (corresponding author), Univ Tokyo, Asian Nat Environm Sci Ctr, Nishitokyo, Tokyo 1880002, Japan.
E-mail Address kobayashi@anesc.u-tokyo.ac.jp; asatone@mail.ecc.u-tokyo.ac.jp; tanengkong@gmail.com; kurokochi@anesc.u-tokyo.ac.jp; asakawa@mail.ecc.u-tokyo.ac.jp; shenkuiliu@nefu.edu.cn; takano@anesc.u-tokyo.ac.jp
ResearcherID Number Liu, Shenkui/AAC-5405-2019
ORCID Number Liu, Shenkui/0000-0001-5294-4765; Kurokochi, Hiroyuki/0000-0003-1691-5718
Funding Agency and Grant Number [25-7241]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [13J07241] Funding Source: KAKEN
Funding Text We are grateful to Professor Masaru Ogasawara at Weed Science Center, Utsunomiya University, for kindly providing the seeds of P. annua. This work was supported by a Grant-in-Aid for JSPS Fellows for S.K. (25-7241).
Times Cited 12
Total Times Cited Count (WoS, BCI, and CSCD) 12
Publisher MDPI
Publisher City BASEL
Publisher Address ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
ISSN 1422-0067
29-Character Source Abbreviation INT J MOL SCI
ISO Source Abbreviation Int. J. Mol. Sci.
Publication Date JAN
Year Published 2015
Volume 16
Issue 1
Beginning Page 496
Ending Page 509
Digital Object Identifier (DOI) 10.3390/ijms16010496
Page Count 14
Web of Science Category Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
Subject Category Biochemistry & Molecular Biology; Chemistry
Document Delivery Number AZ7MN
Unique Article Identifier WOS:000348403100027
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