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Authors Liu, YN; Fu, YB; Coulman, BE
Author Full Name Liu, Yining; Fu, Yong-Bi; Coulman, Bruce E.
Title Evaluating genetic variation and relationships among Puccinellia nuttalliana populations using amplified fragment length polymorphism markers
Source CANADIAN JOURNAL OF PLANT SCIENCE
Language English
Document Type Article
Author Keywords Native grass; Nuttall's alkali grass; genetic variation; amplified fragment length polymorphism
Keywords Plus CORRESPONDING SEED COLLECTIONS; AFLP MARKERS; NATURAL-POPULATIONS; FESTUCA-HALLII; RAPD VARIATION; DIVERSITY; CULTIVARS; SOFTWARE; BLUESTEM; POACEAE
Abstract Nuttall's salt-meadow, or alkali grass [Puccinellia nuttalliana (Shultes) Hitchc.], is a native grass species in North America, well known for its salt tolerance. Little information is available about the genetic diversity of natural populations of this species. Amplified fragment length polymorphism (AFLP) markers were used to examine the inter-population relationships and to compare variances within and among 23 populations collected from the Canadian Great Plains. Five AFLP primer pairs were employed to screen 15 genotypes (five sets of three half-sib plants) from each population, and 185 polymorphic AFLP bands were scored for each sample. The frequencies of these scored bands ranged from 0.02 to 0.99 with a mean of 0.60. The analysis of molecular variance revealed more than 96% of the total AFLP variation resided within populations. Populations were not highly differentiated with only 4% of the total AFLP variation residing among populations. A Mantel test revealed a significant but low correlation between genetic and geographic distances (r =0.29, P =0.024). Implications for P. nuttalliana conservation, germplasm sampling, and cultivar development are discussed.
Author Address [Liu, Yining; Coulman, Bruce E.] Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK S7N 5A8, Canada; [Fu, Yong-Bi] Agr & Agri Food Canada, Plant Gene Resources Canada, Saskatoon Res Ctr, Saskatoon, SK S7N 0X2, Canada
Reprint Address Coulman, BE (corresponding author), Univ Saskatchewan, Dept Plant Sci, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada.
E-mail Address bruce.coulman@usask.ca
Funding Agency and Grant Number Ducks Unlimited Canada
Funding Text We thank Gregory W. Peterson, Carolee Horbach and Vicky Roslinsky at the Agriculture and Agri-Food Canada, Saskatoon Research Centre, for their technical assistance in AFLP analysis. The research was partly supported by Ducks Unlimited Canada.
Times Cited 3
Total Times Cited Count (WoS, BCI, and CSCD) 3
Publisher AGRICULTURAL INST CANADA
Publisher City OTTAWA
Publisher Address 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA
ISSN 0008-4220
29-Character Source Abbreviation CAN J PLANT SCI
ISO Source Abbreviation Can. J. Plant Sci.
Publication Date NOV
Year Published 2013
Volume 93
Issue 6
Beginning Page 1097
Ending Page 1104
Digital Object Identifier (DOI) 10.4141/CJPS2013-157
Page Count 8
Web of Science Category Agronomy; Plant Sciences
Subject Category Agriculture; Plant Sciences
Document Delivery Number 277DY
Unique Article Identifier WOS:000328800400014
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