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| Authors | Liu, YN; Fu, YB; Coulman, BE |
| Author Full Name | Liu, Yining; Fu, Yong-Bi; Coulman, Bruce E. |
| Title | Evaluating genetic variation and relationships among Puccinellia nuttalliana populations using amplified fragment length polymorphism markers |
| Source | CANADIAN JOURNAL OF PLANT SCIENCE |
| Language | English |
| Document Type | Article |
| Author Keywords | Native grass; Nuttall's alkali grass; genetic variation; amplified fragment length polymorphism |
| Keywords Plus | CORRESPONDING SEED COLLECTIONS; AFLP MARKERS; NATURAL-POPULATIONS; FESTUCA-HALLII; RAPD VARIATION; DIVERSITY; CULTIVARS; SOFTWARE; BLUESTEM; POACEAE |
| Abstract | Nuttall's salt-meadow, or alkali grass [Puccinellia nuttalliana (Shultes) Hitchc.], is a native grass species in North America, well known for its salt tolerance. Little information is available about the genetic diversity of natural populations of this species. Amplified fragment length polymorphism (AFLP) markers were used to examine the inter-population relationships and to compare variances within and among 23 populations collected from the Canadian Great Plains. Five AFLP primer pairs were employed to screen 15 genotypes (five sets of three half-sib plants) from each population, and 185 polymorphic AFLP bands were scored for each sample. The frequencies of these scored bands ranged from 0.02 to 0.99 with a mean of 0.60. The analysis of molecular variance revealed more than 96% of the total AFLP variation resided within populations. Populations were not highly differentiated with only 4% of the total AFLP variation residing among populations. A Mantel test revealed a significant but low correlation between genetic and geographic distances (r =0.29, P =0.024). Implications for P. nuttalliana conservation, germplasm sampling, and cultivar development are discussed. |
| Author Address | [Liu, Yining; Coulman, Bruce E.] Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK S7N 5A8, Canada; [Fu, Yong-Bi] Agr & Agri Food Canada, Plant Gene Resources Canada, Saskatoon Res Ctr, Saskatoon, SK S7N 0X2, Canada |
| Reprint Address | Coulman, BE (corresponding author), Univ Saskatchewan, Dept Plant Sci, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada. |
| E-mail Address | bruce.coulman@usask.ca |
| Funding Agency and Grant Number | Ducks Unlimited Canada |
| Funding Text | We thank Gregory W. Peterson, Carolee Horbach and Vicky Roslinsky at the Agriculture and Agri-Food Canada, Saskatoon Research Centre, for their technical assistance in AFLP analysis. The research was partly supported by Ducks Unlimited Canada. |
| Times Cited | 3 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 3 |
| Publisher | AGRICULTURAL INST CANADA |
| Publisher City | OTTAWA |
| Publisher Address | 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA |
| ISSN | 0008-4220 |
| 29-Character Source Abbreviation | CAN J PLANT SCI |
| ISO Source Abbreviation | Can. J. Plant Sci. |
| Publication Date | NOV |
| Year Published | 2013 |
| Volume | 93 |
| Issue | 6 |
| Beginning Page | 1097 |
| Ending Page | 1104 |
| Digital Object Identifier (DOI) | 10.4141/CJPS2013-157 |
| Page Count | 8 |
| Web of Science Category | Agronomy; Plant Sciences |
| Subject Category | Agriculture; Plant Sciences |
| Document Delivery Number | 277DY |
| Unique Article Identifier | WOS:000328800400014 |
| Plants associated with this reference |